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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater

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Author(s):
Medeiros, Raphael Correa [1] ; Daniel, Luiz Antonio [2]
Total Authors: 2
Affiliation:
[1] Univ Fed Santa Maria, Dept Agron & Environm Sci, BR-98400000 Frederico Westphalen, RS - Brazil
[2] Univ Sao Paulo, Sao Carlos Sch Engn, Hydraul & Sanitat Dept, BR-13566590 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF WATER AND HEALTH; v. 13, n. 3, p. 811-818, 2015.
Web of Science Citations: 3
Abstract

More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo) cysts from wastewater samples using ColorSeed (R). Immunomagnetic separation assisted in capturing oocysts mainly in samples with medium and low turbidity. A triple centrifugation method reached recovery rates of 85% and 20%, for Giardia cysts and Cryptosporidium oocysts, respectively, in raw wastewater, and 62.5 and 17.5% in secondary-treated effluent. For low turbidity-treated effluent, membrane filtration reached 67.5% recovery for Giardia cysts and 22.5% for Cryptosporidium oocysts. Simple, quick and low-cost methods do not involve much handling of the samples and could be useful, particularly in developing countries. (AU)

FAPESP's process: 12/50522-0 - Environmental contamination by protozoa Giardia spp. and Cryptosporidium spp. and Ascaris suum: challenges of detection, removal and inactivation of infective forms
Grantee:Regina Maura Bueno Franco
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 10/07902-1 - Assessment of the behavior of protozoa Giardia spp. and Cryptosporidium spp. and indicator microorganisms in the wastewater treatment and in river water
Grantee:Raphael Corrêa Medeiros
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/08653-8 - Removal and inactivation of indicator microorganisms, Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater treatment plants
Grantee:Luiz Antonio Daniel
Support Opportunities: Regular Research Grants