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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Direct electrochemistry of hemoglobin and biosensing for hydrogen peroxide using a film containing silver nanoparticles and poly(amidoamine) dendrimer

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Author(s):
Baccarin, Marina [1, 2] ; Janegitz, Bruno C. [3, 1] ; Berte, Rodrigo [2] ; Vicentini, Fernando Campanha [1] ; Banks, Craig E. [4] ; Fatibello-Filho, Orlando [1] ; Zucolotto, Valtencir [2]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Carlos, Dept Quim, BR-13565970 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Nanomed & Nanotoxicol Grp, BR-13566390 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Ciencias Nat Matemat & Educ, BR-13600970 Araras, SP - Brazil
[4] Manchester Metropolitan Univ, Sch Chem & Environm, Div Chem & Environm Sci, Fac Sci & Engn, Manchester M1 5GD, Lancs - England
Total Affiliations: 4
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 58, p. 97-102, JAN 1 2016.
Web of Science Citations: 34
Abstract

A new architecture for a biosensor is proposed using a glassy carbon electrode (GCE) modified with hemoglobin (Hb) and silver nanopartides (AgNPs) encapsulated in poly(amidoamine) dendrimer (PAMAM). The biosensors were characterized using ultraviolet-visible spectroscopy, zeta-potential and cyclic voltammetry to investigate the interactions between Hb, AgNPs and the PAMAM film. The biosensor exhibited a well-defined cathodic peak attributed to reduction of the Fe3+ present in the heme group in Hb, as revealed by cyclic voltammetry in the presence of O-2. An apparent heterogeneous electron transfer rate of 4.1 s(-1) was obtained. The Hb-AgNPs-PAMAM/GCE third generation biosensor was applied in the amperometric determination of hydrogen peroxide over the linear range from 6.0 x 10(-6) to 9.1 x 10(-5) mol L-1 with a detection limit of 4.9 x 10(-6) mol L-1. The proposed method can be extended to immobilize and evaluate the direct electron transfer of other redox enzymes. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/02877-4 - Development of new biosensors using active Nanoesctrutured materials
Grantee:Bruno Campos Janegitz
Support type: Scholarships in Brazil - Post-Doctorate