Direct electrochemistry of hemoglobin and biosensing for hydrogen peroxide using a film containing silver nanoparticles and poly(amidoamine) dendrimer

Author(s)

Baccarin, Marina ^{[1, 2]} ; Janegitz, Bruno C. ^{[3, 1]} ; Berte, Rodrigo ^[2] ; Vicentini, Fernando Campanha ^[1] ; Banks, Craig E. ^[4] ; Fatibello-Filho, Orlando ^[1] ; Zucolotto, Valtencir ^[2]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Quim, BR-13565970 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Inst Fis Sao Carlos, Nanomed & Nanotoxicol Grp, BR-13566390 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Ciencias Nat Matemat & Educ, BR-13600970 Araras, SP - Brazil

^[4] Manchester Metropolitan Univ, Sch Chem & Environm, Div Chem & Environm Sci, Fac Sci & Engn, Manchester M1 5GD, Lancs - England

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 58, p. 97-102, JAN 1 2016.
• Web of Science Citations: 33

Abstract

A new architecture for a biosensor is proposed using a glassy carbon electrode (GCE) modified with hemoglobin (Hb) and silver nanopartides (AgNPs) encapsulated in poly(amidoamine) dendrimer (PAMAM). The biosensors were characterized using ultraviolet-visible spectroscopy, zeta-potential and cyclic voltammetry to investigate the interactions between Hb, AgNPs and the PAMAM film. The biosensor exhibited a well-defined cathodic peak attributed to reduction of the Fe3+ present in the heme group in Hb, as revealed by cyclic voltammetry in the presence of O-2. An apparent heterogeneous electron transfer rate of 4.1 s(-1) was obtained. The Hb-AgNPs-PAMAM/GCE third generation biosensor was applied in the amperometric determination of hydrogen peroxide over the linear range from 6.0 x 10(-6) to 9.1 x 10(-5) mol L-1 with a detection limit of 4.9 x 10(-6) mol L-1. The proposed method can be extended to immobilize and evaluate the direct electron transfer of other redox enzymes. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 12/02877-4 - Development of New Biosensors Using Active Nanoesctrutured Materials
• Grantee: Bruno Campos Janegitz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral