Immobilization of dapsone in biomimetic systems: self-assembled, Langmuir and Lang...
| Full text | |
| Author(s): |
Baccarin, Marina
[1, 2]
;
Janegitz, Bruno C.
[3, 1]
;
Berte, Rodrigo
[2]
;
Vicentini, Fernando Campanha
[1]
;
Banks, Craig E.
[4]
;
Fatibello-Filho, Orlando
[1]
;
Zucolotto, Valtencir
[2]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Quim, BR-13565970 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Nanomed & Nanotoxicol Grp, BR-13566390 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Ciencias Nat Matemat & Educ, BR-13600970 Araras, SP - Brazil
[4] Manchester Metropolitan Univ, Sch Chem & Environm, Div Chem & Environm Sci, Fac Sci & Engn, Manchester M1 5GD, Lancs - England
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Materials Science & Engineering C-Materials for Biological Applications; v. 58, p. 97-102, JAN 1 2016. |
| Web of Science Citations: | 34 |
| Abstract | |
A new architecture for a biosensor is proposed using a glassy carbon electrode (GCE) modified with hemoglobin (Hb) and silver nanopartides (AgNPs) encapsulated in poly(amidoamine) dendrimer (PAMAM). The biosensors were characterized using ultraviolet-visible spectroscopy, zeta-potential and cyclic voltammetry to investigate the interactions between Hb, AgNPs and the PAMAM film. The biosensor exhibited a well-defined cathodic peak attributed to reduction of the Fe3+ present in the heme group in Hb, as revealed by cyclic voltammetry in the presence of O-2. An apparent heterogeneous electron transfer rate of 4.1 s(-1) was obtained. The Hb-AgNPs-PAMAM/GCE third generation biosensor was applied in the amperometric determination of hydrogen peroxide over the linear range from 6.0 x 10(-6) to 9.1 x 10(-5) mol L-1 with a detection limit of 4.9 x 10(-6) mol L-1. The proposed method can be extended to immobilize and evaluate the direct electron transfer of other redox enzymes. (C) 2015 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 12/02877-4 - Development of new biosensors using active Nanoesctrutured materials |
| Grantee: | Bruno Campos Janegitz |
| Support type: | Scholarships in Brazil - Post-Doctorate |