| Full text | |
| Author(s): |
Pedro L. R. da Cruz
[1]
;
Leila R. Giarola
[2]
;
Suellen da Silva Moraes
[3]
;
Déborah Ellen S. G. da Silva
[4]
;
Joelma Marcon
[5]
;
João L. Azevedo
[6]
;
Welington L. Araújo
[7]
;
Luciana G. de Oliveira,
[8]
Total Authors: 8
|
| Affiliation: | [1] Universidade Estadual de Campinas. Instituto de Química. Departamento de Química Orgânica - Brasil
[2] Universidade Estadual de Campinas. Faculdade de Farmácia - Brasil
[3] Universidade Estadual de Campinas. Instituto de Química. Departamento de Química Orgânica - Brasil
[4] Universidade Estadual de Campinas. Instituto de Química. Departamento de Química Orgânica - Brasil
[5] Universidade de São Paulo. Escola de Agricultura Luiz de Queiroz. Departamento de Genética - Brasil
[6] Universidade de São Paulo. Escola de Agricultura Luiz de Queiroz. Departamento de Genética - Brasil
[7] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia - Brasil
[8] Universidade de São Paulo. Escola de Agricultura Luiz de Queiroz. Departamento de Genética - Brasil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | Química Nova; v. 38, n. 3, p. 333-341, 2015-03-00. |
| Abstract | |
Polyketides and non-ribosomal peptides are natural products widely found in bacteria, fungi and plants. The biological activities associated with these metabolites have attracted special attention in biopharmaceutical studies. Polyketide synthases act similarly to fatty acids synthetases and the whole multi-enzymatic set coordinating precursor and extending unit selection and reduction levels during chain growth. Acting in a similarly orchestrated model, non-ribosomal peptide synthetases biosynthesize NRPs. PKSs-I and NRPSs enzymatic modules and domains are collinearly organized with the parent gene sequence. This arrangement allows the use of degenerated PCR primers to amplify targeted regions in the genes corresponding to specific enzymatic domains such as ketosynthases and acyltransferases in PKSs and adenilation domains in NRPSs. Careful analysis of these short regions allows the classifying of a set of organisms according to their potential to biosynthesize PKs and NRPs. In this work, the biosynthetic potential of a set of 13 endophytic actinobacteria from Citrus reticulata for producing PKs and NRP metabolites was evaluated. The biosynthetic profile was compared to antimicrobial activity. Based on the inhibition promoted, 4 strains were considered for cluster analysis. A PKS/NRPS phylogeny was generated in order to classify some of the representative sequences throughout comparison with homologous genes. Using this approach, a molecular fingerprint was generated to help guide future studies on the most promising strains. (AU) | |
| FAPESP's process: | 09/03793-6 - Mining the Biosynthetic Potential of Streptomycetes: Comparison using S. coelicolor as a model strain |
| Grantee: | Pedro Luís Rocha da Cruz |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 10/16798-3 - Exploring Brazilian biodiversity to obtain 'non-natural' natural products |
| Grantee: | Suéllen da Silva Moraes |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 08/00605-1 - Exploring Brazilian biodiversity to obtain 'non-natural' natural products |
| Grantee: | Luciana Gonzaga de Oliveira |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 08/06097-8 - Exploring Brazilian biodiversity to obtain 'non-natural' natural products |
| Grantee: | Leila Regina Giarola |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |