| Full text | |
| Author(s): |
Bertol, Jessica Wildgrube
[1]
;
Duarte Fregolente, Maria Clara
[1, 2]
;
Ramos Caruzo, Thabata Alessandra
[1]
;
da Silva, Marcio Jose
[3]
;
Munford, Veridiana
[4]
;
Palazzi Safadi, Marco Aurelio
[5]
;
Racz, Maria Lucia
[4]
;
Viccari Gatti, Maria Silvia
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Campinas, SP - Brazil
[2] Univ Cidade Sao Paulo, Sao Paulo, SP - Brazil
[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil
[4] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo, SP - Brazil
[5] Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Memórias do Instituto Oswaldo Cruz; v. 110, n. 6, p. 786-792, SEP 2015. |
| Web of Science Citations: | 0 |
| Abstract | |
Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation. (AU) | |
| FAPESP's process: | 11/13725-8 - Full genomic analysis of human group A rotavirus G2 and non-typeable genotype G strains from São Paulo, Brazil: strains classification using new system and variability investigation due to vaccine introduction in the Brazilian Public Health System |
| Grantee: | Maria Silvia Viccari Gatti |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 09/08685-7 - Full genomic analysis of human group A rotavirus G2 and non-typable genotype G strains from São Paulo, Brazil: strains classification using new system and variability investigation due to vaccine introduction in the Brazilian public health system. |
| Grantee: | Thabata Alessandra Ramos Caruzo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |