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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Green Tea Extract Rich in Epigallocatechin-3-Gallate Prevents Fatty Liver by AMPK Activation via LKB1 in Mice Fed a High-Fat Diet

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Author(s):
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Santamarina, Aline B. [1] ; Oliveira, Juliana L. [1] ; Silva, Fernanda P. [1] ; Carnier, June [1] ; Mennitti, Lais V. [2] ; Santana, Aline A. [1] ; de Souza, Gabriel H. I. [3] ; Ribeiro, Eliane B. [1] ; Oller do Nascimento, Claudia M. [1] ; Lira, Fabio S. [4] ; Oyama, Lila M. [1]
Total Authors: 11
Affiliation:
[1] Univ Fed Sao Paulo, Dept Fisiol, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, Santos - Brazil
[3] Univ Sao Judas Tadeu, Lab Movimento Humano, Sao Paulo - Brazil
[4] Univ Estadual Paulista, UNESP, Dept Phys Educ, Exercise & Immunometab Res Grp, Presidente Prudente, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 10, n. 11 NOV 4 2015.
Web of Science Citations: 29
Abstract

Supplementation with epigallocatechin-3-gallate has been determined to aid in the prevention of obesity. Decaffeinated green tea extract appears to restore a normal hepatic metabolic profile and attenuate high-fat diet (HFD)-induced effects, thereby preventing nonalcoholic fatty liver disease in mice. Mice were maintained on either a control diet (CD) or HFD for 16 weeks and supplemented with either water or green tea extract (50 mg/kg/day). The body mass increase, serum adiponectin level, and lipid profile were measured over the course of the treatment. Furthermore, the AMPK pathway protein expression in the liver was measured. From the fourth week, the weight gain in the CD + green tea extract (CE) group was lower than that in the CD + water (CW) group. From the eighth week, the weight gain in the HFD + water (HFW) group was found to be higher than that in the CW group. Moreover, the weight gain in the HFD + green tea extract (HFE) group was found to be lower than that in the HFW group. Carcass lipid content was found to be higher in the HFW group than that in the CW and HFE groups. Serum analysis showed reduced non-esterified fatty acid level in the CE and HFE groups as compared with their corresponding placebo groups. Increased adiponectin level was observed in the same groups. Increased VLDL-TG secretion was observed in the HFW group as compared with the CW and HFE groups. Increased protein expression of AdipoR2, SIRT1, pLKB1, and pAMPK was observed in the HFE group, which explained the reduced expression of ACC, FAS, SREBP-1, and ChREBP in this group. These results indicate that the effects of decaffeinated green tea extract may be related to the activation of AMPK via LKB1 in the liver of HFD-fed mice. (AU)

FAPESP's process: 14/19508-7 - Effect of green tea extract rich in epigallocatechin-3-gallate on hepatic metabolic biomarkers in mice treated with high fat diet
Grantee:Lila Missae Oyama
Support Opportunities: Regular Research Grants