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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods

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Author(s):
Cadioli, Fabiano Antonio [1] ; Fidelis Junior, Otavio Luiz [2] ; Sampaio, Paulo Henrique [2] ; dos Santos, Giuliana Nascimento [1] ; Andre, Marcos Rogerio [2] ; Garcia de Almeida Castilho, Kayo Jose [1] ; Machado, Rosangela Zacarias [2]
Total Authors: 7
Affiliation:
[1] UNESP Aracatuba, FMVA, BR-16050470 Aracatuba, SP - Brazil
[2] UNESP Jaboticabal, FCAV, BR-14884900 Jaboticabal, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Veterinary Parasitology; v. 214, n. 1-2, p. 174-177, NOV 30 2015.
Web of Science Citations: 2
Abstract

Trypanosoma vivax affects cattle herds in Africa and Americas and has been spreading rapidly in Brazil, through introduction of animals with subclinical infections and without apparent parasitemia, which makes its diagnosis challenging. PCR and LAMP are effective in detecting the presence of T. vivax DNA in situations of low parasitemia. LAMP is simpler and faster technique than PCR, and can be performed in the field, with limited resources. In this study, the capacities of conventional PCR and LAMP for detecting T. vivax in bovine blood samples classified as aparasitemic were evaluated. The capacity of conventional PCR (56.25%) for detecting positive samples was lower than that of LAMP (93.73%). This may influence the choice of screening tests for cattle herds infected with T. vivax. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/04066-9 - Detection of Trypanosoma vivax in bovines experimentally infected using lamp and PCR during apparent aparasitemic phases
Grantee:Giuliana Nascimento dos Santos
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/02284-3 - Hematological, biochemical, sorologic and histopathological changes of naturally and experimentally infected bovines by Trypanosoma vivax
Grantee:Fabiano Antonio Cadioli
Support Opportunities: Regular Research Grants
FAPESP's process: 11/15945-5 - PATHOGENICITY OF "LINS" STRAIN OF Trypanosoma vivax IN NATURALLY AND EXPERIMENTALLY INFECTED CATTLE
Grantee:Otávio Luiz Fidelis Júnior
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/11600-1 - Serological and molecular techniques assessing the treatment effectiveness against T. vivax in experimentally infected goats
Grantee:Paulo Henrique Sampaio
Support Opportunities: Scholarships in Brazil - Doctorate