Plasma anti-Mtillerian hormone as a predictive endocrine marker to select Bos taurus (Holstein) and Bos indicus (Nelore) calves for in vitro embryo production

Author(s):Show less -

Batista, E. O. S. ^[1] ; Guerreiro, B. M. ^[1] ; Freitas, B. G. ^[1] ; Silva, J. C. B. ^[2] ; Vieira, L. M. ^[1] ; Ferreira, R. M. ^[3] ; Rezende, R. G. ^[1] ; Basso, A. C. ^[4] ; Lopes, R. N. V. R. ^[5] ; Renno, F. P. ^[6] ; Souza, A. H. ^[7] ; Baruselli, P. S. ^[1]

Affiliation

^[1] FMVZ USP, Dept Anim Reprod, Sao Paulo - Brazil

^[2] Embryo Sys, Ouro Fino, MG - Brazil

^[3] Univ Sao Paulo, Dept Vet Med, Fac Anim Sci & Food Engn, Pirassununga - Brazil

^[4] In Vitro Brasil Ltda, Mogi Mirim, SP - Brazil

^[5] Genese Co, Sao Paulo, SP - Brazil

^[6] FMVZ USP Campus, Dept Anim Prod & Nutr, Pirassununga, SP - Brazil

^[7] Ceva Sante Anim, Libourne, Gironde - France

• Document type: Journal article
• Source: DOMESTIC ANIMAL ENDOCRINOLOGY; v. 54, p. 1-9, JAN 2016.
• Web of Science Citations: 13

Abstract

This study evaluated the association between plasma anti-Mullerian hormone (AMH) concentrations and in vitro embryo production in Bos indicus (Nelore; experiment 1) and Bos taurus (Holstein; experiment 2) calves superstimulated or not with 140 mg of porcine follicle-stimulating hormone (pFSH; 4 decreasing doses twice daily). Oocytes were recovered from calves aged 2 to 4 mo after receiving gonadotropin stimulation (Nelore, n = 15; Holstein, n = 12) or not (Nelore, n = 15; Holstein, n = 12). Cycling heifers formed a positive control group (n = 15 for Nelore {[}aged 18-24 mo], n = 10 for Holstein {[}aged 1416 mo]). All the calves underwent laparoscopic ovum pickup, and cycling heifers underwent a regular transvaginal ultrasound-guided ovum pickup for oocyte recovery. Immediately before oocyte retrieval, blood samples were taken for subsequent AMH determination (ng/mL). Regardless of the genetic group, calves that received pFSH (3.6 +/- 1.1 in Nelore and 4.6 +/- 1.2 in Holstein) or did not receive pFSH (3.2 +/- 1.0 in Nelore and 2.5 +/- 0.8 in Holstein) had greater plasma AMH concentrations (P = 0.01 in Nelore and P = 0.003 in Holstein) than cycling heifers (1.1 +/- 0.2 in Nelore and 0.6 +/- 0.07 in Holstein). AMH concentrations in calves with or without pFSH were similar in both genetic groups (3.6 +/- 1.1 vs 3.2 +/- 1.0 in Nelore; 4.6 +/- 1.2 vs 2.5 +/- 0.8 in Holstein). In calves, positive correlations were observed between plasma AMH concentrations and the numbers of follicles >2 mm (r = 0.86, P < 0.0001 in Nelore; r = 0.78, P < 0.0001 in Holstein), cumulus-oocyte complexes (COCs) retrieved (r = 0.91, P < 0.0001 in Nelore; r = 0.82, P < 0.0001 in Holstein), COCs cultured (r = 0.71, P < 0.0001 in Nelore; r = 0.79, P < 0.0001 in Holstein), and blastocysts produced (r = 0.62, P = 0.0003 in Nelore; r = 0.58, P = 0.009 in Holstein), and these results were independent of pFSH treatment. In conclusion, calves had greater plasma AMH concentrations than cycling heifers. In addition, treatment with pFSH did not influence AMH concentrations in calves, regardless of the genetic group. More importantly, plasma AMH concentrations were positively correlated with the antral follicle population and the number of COCs retrieved, COCs cultured, and blastocysts produced in B indicus and B taurus calves. Therefore, AMH is a promising tool for selecting oocyte donor calves to maximize results during in vitro embryo production. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 13/50827-9 - Effects of maternal donor age on oocyte quality and in vitro embryo production
• Grantee: Bruno Valente Sanches
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE