| Full text | |
| Author(s): |
Rodrigues, Gabriela Silva
[1]
;
Godinho, Rosely Oliveira
[2]
;
Kiyomoto, Beatriz Hitomi
[1]
;
Gamba, Juliana
[1]
;
Bulle Oliveira, Acary Souza
[1]
;
Schmidt, Beny
[3]
;
Tengan, Celia Harumi
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Neurol & Neurosurg, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Div Cellular Pharmacol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Pathol, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 6, FEB 9 2016. |
| Web of Science Citations: | 2 |
| Abstract | |
Nitric oxide (NO) is an important signaling messenger involved in different mitochondrial processes but only few studies explored the participation of NO in mitochondrial abnormalities found in patients with genetic mitochondrial deficiencies. In this study we verified whether NO synthase (NOS) activity was altered in different types of mitochondrial abnormalities and whether changes in mitochondrial function and NOS activity could be associated with the induction of apoptosis. We performed a quantitative and integrated analysis of NOS activity in individual muscle fibres of patients with mitochondrial diseases, considering mitochondrial function (cytochrome-c-oxidase activity), mitochondrial content, mitochondrial DNA mutation and presence of apoptotic nuclei. Our results indicated that sarcolemmal NOS activity was increased in muscle fibres with mitochondrial proliferation, supporting the relevance of neuronal NOS in the mitochondrial biogenesis process. Sarcoplasmic NOS activity was reduced in cytochrome-c-oxidase deficient fibres, probably as a consequence of the involvement of NO in the regulation of the respiratory chain. Alterations in NOS activity or mitochondrial abnormalities were not predisposing factors to apoptotic nuclei. Taken together, our results show that NO can be considered a potential molecular target for strategies to increase mitochondrial content and indicate that this approach may not be associated with increased apoptotic events. (AU) | |
| FAPESP's process: | 07/00808-7 - In situ and in vitro analysis of nitric oxide, apoptosis and mitochondrial biogenesis in mitochondrial diseases and C2C12 myoblast cell line |
| Grantee: | Gabriela Silva Rodrigues |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 07/03145-9 - Study of the relationship between nitric oxide synthesis, mitochondrial biogenesis and apoptosis in skeletal muscle |
| Grantee: | Célia Harumi Tengan |
| Support Opportunities: | Regular Research Grants |