| Full text | |
| Author(s): |
Pereira, Rubner Goncalves
[1]
;
Garcia, Vera Lucia
[2]
;
Nova Rodrigues, Marili Villa
[2]
;
Martinez, Julian
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Campinas UNICAMP, Coll Food Engn FEA, Food Engn Dept DEA, Lab High Pressure Food Engn LAPEA, BR-13083862 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Chem Biol & Agr Res Ctr CPQBA, BR-13148218 Paulinia, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Separation and Purification Technology; v. 158, p. 204-211, JAN 28 2016. |
| Web of Science Citations: | 8 |
| Abstract | |
Extracts rich in lignans were obtained from Phyllanthus amarus by Pressurized Liquid Extraction (PLE). The influences of temperature and solvent type on the extraction yield and chemical composition of the extracts were evaluated. Temperature was evaluated from 35 degrees C to 80 degrees C and the used solvents were: water, ethanol and a mixture of ethanol + water (50% v/v). All extractions were performed at the pressure of 10 MPa. A low pressure extraction method (LPE) using the Ultra-Turrax disperser and the same solvents, at room temperature and atmospheric pressure, was performed to compare the results with those of PLE. The extracts were analyzed by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and seven lignans were identified: 5-demethoxy-niranthin, phyllanthin, filtetralin, 5-demethoxy-nirtetralin, nirtetralin, hipophyllanthin and niranthin. High Performance Liquid Chromatography (HPLC) was used to quantify the most bioactive lignans, phyllanthin and niranthin, in the extracts. In the PLE method, the effect of temperature was negligible in the studied range for the recovery of these lignans. The binary mixture ethanol + water (50% v/v) was the best solvent for the recovery of high amounts of both lignans, independently of the extraction method. However, PLE with 100% ethanol provided the most concentrated extracts in both lignans. PLE achieved higher global extraction yield than in LPE, but lower lignan recoveries. The contents of the major lignans extracted by each method varied significantly, suggesting that phyllanthin and niranthin are located in different parts of the plant tissue. The selection of the best method to extract lignans form P. amarus depends on the operation costs. (C) 2015 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 13/02203-6 - Development of equipments for extraction, particle formation and chemical reactions using pressurized fluids |
| Grantee: | Julian Martínez |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/22119-7 - Study of the decomposition mechanisms of the plant cell wall |
| Grantee: | Camila Alves de Rezende |
| Support Opportunities: | Regular Research Grants |