Development of equipments for extraction, particle formation and chemical reactions using pressurized fluids

• Grant number: 13/02203-6
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2013
• End date: July 31, 2015
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Julian Martínez
• Grantee: Julian Martínez
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Camila Alves de Rezende ; Maria Angela de Almeida Meireles Petenate ; Maurício Ariel Rostagno

Abstract

This project is dedicated to the construction of new equipments for the processing of natural products using high pressure fluids, such as carbon dioxide (CO2) and water. The use of pressurized solvents is an alternative to toxic organic solvents, or to techniques that require high temperatures. The equipments to be built will address the extraction and nanoparticle formation of bioactive compounds from raw materials or food industry byproducts, both of vegetal sources, and the synthesis of esters by means of reactions with the possibility of using enzymes. In the extraction step supercritical CO2 (SFE) and pressurized water (PLE) extractions will be performed. Indeed, ultrasound will be applied during the extractions in order to accelerate the processes and increase their yields. The extracts obtained by SFE and PLE, with or without ultrasound, will be lead to encapsulation procedures, which will be performed through methods that use supercritical CO2 as antisolvent (SAS and SFEE). The produced particles will be evaluated in terms of their size, uniformity, shape, concentration and stability of their bioactive compounds. The kinetics of controlled release of the compounds will be evaluated, as well as their stability in the liberation mean. Finally, the biological activities of the encapsulated products will be evaluated by specific tests. The synthesis of esters will be performed using supercritical CO2 as reaction medium, alcohols, sugars, fatty acids and terpenes as substrates, and immobilized lipases ad catalyzers, leading to sugar or terpenic esters as products. The processes of extraction, particle formation and release will have their mechanisms studied by means of mathematical modeling, dealing with thermodynamic and kinetic issues. The performing of the proposed processes will demand the construction of four equipments, being one of supercritical extraction (SFE) and pressurized liquid extraction (PLE), one of SFE and PLE assisted by ultrasound, one for particle formation by SAS and SFEE, and a high pressure reactor. Therefore, the financial resources of the present proposal concentrate in equipments, consumables and services needed to build and operate those units. (AU)

Scientific publications

(28)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE AGUIAR, ANA CAROLINA; DOS SANTOS, PHILIPE; COUTINHO, JANCLEI PEREIRA; BARBERO, GERARDO FERNANDEZ; GODOY, HELENA TEIXEIRA; MARTINEZ, JULIAN. Supercritical fluid extraction and low pressure extraction of Biquinho pepper (Capsicum chinense). LWT-FOOD SCIENCE AND TECHNOLOGY, v. 59, n. 2, 2, SI, p. 1239-1246, DEC 2014. (13/02203-6)

DA FONSECA MACHADO, ANA PAULA; PASQUEL-REATEGUI, JOSE LUIS; FERNANDEZ BARBERO, GERARDO; MARTINEZ, JULIAN. Pressurized liquid extraction of bioactive compounds from blackberry (Rubus fruticosus L.) residues: a comparison with conventional methods. Food Research International, v. 77, n. 3, p. 675-683, NOV 2015. (13/02203-6, 11/08656-7)

SOARES, JULIANA F.; DAL PRA, VALERIA; BARRALES, FRANCISCO MANUEL; DOS SANTOS, PHILIPE; KUHN, RAQUEL C.; REZENDE, CAMILA A.; MARTINEZ, JULIAN; MAZUTTI, MARCIO A.. EXTRACTION OF RICE BRAN OIL USING SUPERCRITICAL CO2 COMBINED WITH ULTRASOUND. Brazilian Journal of Chemical Engineering, v. 35, n. 2, p. 785-794, APR-JUN 2018. (13/02203-6)

DOS SANTOS, PHILIPE; ZABOT, GIOVANI L.; MEIRELES, M. ANGELA A.; MAZUTTI, MARCIO A.; MARTINEZ, JULIAN. Synthesis of eugenyl acetate by,enzymatic reactions in supercritical carbon dioxide. Biochemical Engineering Journal, v. 114, p. 1-9, OCT 15 2016. (09/54137-1, 15/11932-7, 13/02203-6)

DOS SANTOS, PHILIPE; DE AGUIAR, ANA C.; VIGANO, JULIANE; BOEING, JOANA SCHUELTER; VISENTAINER, JESUI V.; MARTINEZ, JULIAN. Supercritical CO2 extraction of cumbaru oil (Dipteryx alata Vogel) assisted by ultrasound: Global yield, kinetics and fatty acid composition. JOURNAL OF SUPERCRITICAL FLUIDS, v. 107, p. 75-83, JAN 2016. (14/00372-8, 13/02203-6)

DOS SANTOS, PHILIPE; REZENDE, CAMILA A.; MARTINEZ, JULIAN. Activity of immobilized lipase from Candida antarctica (Lipozyme 435) and its performance on the esterification of oleic acid in supercritical carbon dioxide. JOURNAL OF SUPERCRITICAL FLUIDS, v. 107, p. 170-178, JAN 2016. (13/02203-6)

PAES, JULIANA; DOTTA, RAQUEL; BARBERO, GERARDO F.; MARTINEZ, JULIAN. Extraction of phenolic compounds and anthocyanins from blueberry (Vaccinium myrtillus L.) residues using supercritical CO2 and pressurized liquids. JOURNAL OF SUPERCRITICAL FLUIDS, v. 95, p. 8-16, NOV 2014. (13/02203-6)

BARRALES, FRANCISCO MANUEL; REZENDE, CAMILA ALVES; MARTINEZ, JULIAN. Supercritical CO2 extraction of passion fruit (Passiflora edulis sp.) seed oil assisted by ultrasound. JOURNAL OF SUPERCRITICAL FLUIDS, v. 104, p. 183-192, SEP 2015. (13/02203-6, 12/22119-7)

PASQUEL REATEGUI, JOSE LUIS; DA FONSECA MACHADO, ANA PAULA; BARBERO, GERARDO F.; REZENDE, CAMILA A.; MARTINEZ, JULIAN. Extraction of antioxidant compounds from blackberry (Rubus sp.) bagasse using supercritical CO2 assisted by ultrasound. JOURNAL OF SUPERCRITICAL FLUIDS, v. 94, p. 223-233, OCT 2014. (13/02203-6, 12/22119-7)

DA FONSECA MACHADO, ANA PAULA; DUARTE PEREIRA, ANA LUIZA; FERNANDEZ BARBERO, GERARDO; MARTINEZ, JULIAN. Recovery of anthocyanins from residues of Rubus fruticosus, Vaccinium myrtillus and Eugenia brasiliensis by ultrasound assisted extraction, pressurized liquid extraction and their combination. Food Chemistry, v. 231, p. 1-10, SEP 15 2017. (15/11932-7, 13/02203-6)

VIGANO, JULIANE; AGUIAR, ANA C.; MORAES, DAMILA R.; JARA, JOSE L. P.; EBERLIN, MARCOS N.; CAZARIN, CINTHIA B. B.; MAROSTICA JUNIOR, MARIO R.; MARTINEZ, JULIAN. Sequential high pressure extractions applied to recover piceatannol and scirpusin B from passion fruit bagasse. Food Research International, v. 85, p. 51-58, JUL 2016. (14/00372-8, 13/02203-6)

GARCIA-MENDOZA, MARIA DEL PILAR; ESPINOSA-PARDO, FABER ARIEL; BASEGGIO, ANDRESSA MARA; FERNANDEZ BARBERO, GERARDO; MAROSTICA JUNIOR, MARIO ROBERTO; ROSTAGNO, MAURICIO ARIEL; MARTINEZ, JULIAN. Extraction of phenolic compounds and anthocyanins from jucara (Euterpe edulis Mart.) residues using pressurized liquids and supercritical fluids. JOURNAL OF SUPERCRITICAL FLUIDS, v. 119, p. 9-16, JAN 2017. (15/11932-7, 13/04304-4, 13/02203-6)

SOARES, JULIANA F.; DAL PRA, VALERIA; BARRALES, FRANCISCO MANUEL; DOS SANTOS, PHILIPE; KUHN, RAQUEL C.; REZENDE, CAMILA A.; MARTINEZ, JULIAN; MAZUTTI, MARCIO A.. EXTRACTION OF RICE BRAN OIL USING SUPERCRITICAL CO2 COMBINED WITH ULTRASOUND. Brazilian Journal of Chemical Engineering, v. 35, n. 2, p. 10-pg., 2018-04-01. (13/02203-6)

VIGANO, JULIANE; DA FONSECA MACHADO, ANA PAULA; MARTINEZ, JULIAN. Sub- and supercritical fluid technology applied to food waste processing. JOURNAL OF SUPERCRITICAL FLUIDS, v. 96, p. 15-pg., 2015-01-01. (13/02203-6, 14/00372-8)

DE AGUIAR, ANA CAROLINA; DOS SANTOS, PHILIPE; COUTINHO, JANCLEI PEREIRA; BARBERO, GERARDO FERNANDEZ; GODOY, HELENA TEIXEIRA; MARTINEZ, JULIAN. Supercritical fluid extraction and low pressure extraction of Biquinho pepper (Capsicum chinense). LWT-FOOD SCIENCE AND TECHNOLOGY, v. 59, n. 2, p. 8-pg., 2014-12-01. (13/02203-6)

DA FONSECA MACHADO, ANA PAULA; REZENDE, CAMILA ALVES; RODRIGUES, RODNEY ALEXANDRE; FERNANDEZ BARBERO, GERARDO; VIEIRA E ROSA, PAULO DE TARSO; MARTINEZ, JULIAN. Encapsulation of anthocyanin-rich extract from blackberry residues by spray-drying, freeze-drying and supercritical antisolvent. Powder Technology, v. 340, p. 553-562, DEC 2018. (16/13602-7, 15/11932-7, 13/02203-6)

BAIAO DIAS, ARTHUR LUIZ; ARROIO SERGIO, CAMILLA SCARELLI; SANTOS, PHILIPE; BARBERO, GERARDO FERNANDEZ; REZENDE, CAMILA ALVES; MARTINEZ, JULIAN. Ultrasound-assisted extraction of bioactive compounds from dedo de moca pepper (Capsicum baccatum L.): Effects on the vegetable matrix and mathematical modeling. Journal of Food Engineering, v. 198, p. 36-44, APR 2017. (15/11932-7, 13/02203-6)

ESPINOSA-PARDO, FABER ARIEL; NAKAJIMA, VANIA MAYUVNI; MACEDO, GABRIELA ALVES; MACEDO, JULIANA ALVES; MARTINEZ, JULIAN. Extraction of phenolic compounds from dry and fermented orange pomace using supercritical CO2 and cosolvents. FOOD AND BIOPRODUCTS PROCESSING, v. 101, p. 1-10, JAN 2017. (15/11932-7, 13/02203-6)

VIGANO, JULIANE; ZABOT, GIOVANI L.; MARTINEZ, JULIAN. Supercritical fluid and pressurized liquid extractions of phytonutrients from passion fruit by-products: Economic evaluation of sequential multi-stage and single-stage processes. JOURNAL OF SUPERCRITICAL FLUIDS, v. 122, p. 88-98, APR 2017. (14/00372-8, 15/11932-7, 13/02203-6)

DE AGUIAR, ANA CAROLINA; COUTINHO, JANCLEI PEREIRA; FERNANDEZ BARBERO, GERARDO; GODOY, HELENA TEIXEIRA; MARTINEZ, JULIAN. Comparative Study of Capsaicinoid Composition in Capsicum Peppers Grown in Brazil. INTERNATIONAL JOURNAL OF FOOD PROPERTIES, v. 19, n. 6, p. 1292-1302, JUN 2 2016. (11/08656-7, 13/02203-6)

VIGANO, JULIANE; BRUMER, ISABELA ZABOTI; DE CAMPOS BRAGA, PATRICIA APARECIDA; DA SILVA, JULIANA KELLY; MAROSTICA JUNIOR, MARIO ROBERTO; REYES REYES, FELIX GUILLERMO; MARTINEZ, JULIAN. Pressurized liquids extraction as an alternative process to readily obtain bioactive compounds from passion fruit rinds. FOOD AND BIOPRODUCTS PROCESSING, v. 100, n. A, p. 382-390, OCT 2016. (14/00372-8, 15/11932-7, 13/02203-6)

VIGANO, JULIANE; DA FONSECA MACHADO, ANA PAULA; MARTINEZ, JULIAN. Sub- and supercritical fluid technology applied to food waste processing. JOURNAL OF SUPERCRITICAL FLUIDS, v. 96, n. SI, p. 272-286, JAN 2015. (14/00372-8, 13/02203-6)

DE AGUIAR, ANA CAROLINA; SALES SILVA, LUIZ PAULO; DE REZENDE, CAMILA ALVES; BARBERO, GERARDO FERNANDEZ; MARTINEZ, JULIAN. Encapsulation of pepper oleoresin by supercritical fluid extraction of emulsions. JOURNAL OF SUPERCRITICAL FLUIDS, v. 112, p. 37-43, JUN 2016. (11/08656-7, 13/02203-6, 12/22119-7)

PEREIRA, RUBNER GONCALVES; GARCIA, VERA LUCIA; NOVA RODRIGUES, MARILI VILLA; MARTINEZ, JULIAN. Extraction of lignans from Phyllanthus amarus Schum. & Thonn using pressurized liquids and low pressure methods. Separation and Purification Technology, v. 158, p. 204-211, JAN 28 2016. (13/02203-6, 12/22119-7)

VIGANO, JULIANE; COUTINHO, JANCLEI P.; SOUZA, DANILO S.; BARONI, NAIARA A. F.; GODOY, HELENA T.; MACEDO, JULIANA A.; MARTINEZ, JULIAN. Exploring the selectivity of supercritical CO2 to obtain nonpolar fractions of passion fruit bagasse extracts. JOURNAL OF SUPERCRITICAL FLUIDS, v. 110, p. 1-10, APR 2016. (14/00372-8, 13/02203-6)

BAIAO DIAS, ARTHUR LUIZ; ARROIO SERGIO, CAMILLA SCARELLI; SANTOS, PHILIPE; BARBERO, GERARDO FERNANDEZ; REZENDE, CAMILA ALVES; MARTINEZ, JULIAN. Effect of ultrasound on the supercritical CO2 extraction of bioactive compounds from dedo de moca pepper (Capsicum baccatum L. var. pendulum). ULTRASONICS SONOCHEMISTRY, v. 31, p. 284-294, JUL 2016. (13/02203-6)

VIGANO, JULIANE; BRUMER, ISABELA ZABOTI; DE CAMPOS BRAGA, PATRICIA APARECIDA; DA SILVA, JULIANA KELLY; MAROSTICA JUNIOR, MARIO ROBERTO; REYES REYES, FELIX GUILLERMO; MARTINEZ, JULIAN. Pressurized liquids extraction as an alternative process to readily obtain bioactive compounds from passion fruit rinds. FOOD AND BIOPRODUCTS PROCESSING, v. 100, p. 9-pg., 2016-10-01. (14/00372-8, 13/02203-6, 15/11932-7)

DA FONSECA MACHADO, ANA PAULA; PASQUEL-REATEGUI, JOSE LUIS; FERNANDEZ BARBERO, GERARDO; MARTINEZ, JULIAN. Pressurized liquid extraction of bioactive compounds from blackberry (Rubus fruticosus L.) residues: a comparison with conventional methods. Food Research International, v. 77, p. 9-pg., 2015-11-01. (13/02203-6, 11/08656-7)

Filed patent(s) as a result of this research project