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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Streptococcus mutans Can Modulate Biofilm Formation and Attenuate the Virulence of Candida albicans

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Author(s):
Barbosa, Junia Oliveira [1] ; Rossoni, Rodnei Dennis [1] ; Godinho Vilela, Simone Furgeri [1] ; de Alvarenga, Janana Araujo [1] ; Velloso, Marisol dos Santos [1] ; de Azevedo Prata, Marcia Cristina [2] ; Cardoso Jorge, Antonio Olavo [1] ; Junqueira, Juliana Campos [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista UNESP, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil
[2] Embrapa Gado Leite, Juiz De Fora, MG - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 11, n. 3 MAR 2 2016.
Web of Science Citations: 19
Abstract

Streptococcus mutans and Candida albicans are found together in the oral biofilms on dental surfaces, but little is known about the ecological interactions between these species. Here, we studied the effects of S. mutans UA159 on the growth and pathogencity of C. albicans. Initially, the effects of S. mutans on the biofilm formation and morphogenesis of C. albicans were tested in vitro. Next, we investigate the influence of S. mutans on pathogenicity of C. albicans using in vivo host models, in which the experimental candidiasis was induced in G. mellonella larvae and analyzed by survival curves, C. albicans count in hemolymph, and quantification of hyphae in the host tissues. In all the tests, we evaluated the direct effects of S. mutans cells, as well as the indirect effects of the subproducts secreted by this microorganism using a bacterial culture filtrate. The in vitro analysis showed that S. mutans cells favored biofilm formation by C. albicans. However, a reduction in biofilm viable cells and inhibition of hyphal growth was observed when C. albicans was in contact with the S. mutans culture filtrate. In the in vivo study, injection of S. mutans cells or S. mutans culture filtrate into G. mellonella larvae infected with C. albicans increased the survival of these animals. Furthermore, a reduction in hyphal formation was observed in larval tissues when C. albicans was associated with S. mutans culture filtrate. These findings suggest that S. mutans can secrete subproducts capable to inhibit the biofilm formation, morphogenesis and pathogenicity of C. albicans, attenuating the experimental candidiasis in G. mellonella model. (AU)

FAPESP's process: 12/15262-8 - Interaction between Streptococcus mutans and Candida albicans: formation of biofilm in vitro and experimental study on models of invertebrates
Grantee:Júnia Oliveira Barbosa
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 12/19915-6 - Interaction between Streptococcus mutans and Lactobacillus acidophilus with Candida albicans: biofilm formation in vitro and experimental study in invertebrate models
Grantee:Juliana Campos Junqueira
Support Opportunities: Regular Research Grants