The effects of the mycotoxin austdiol on cell cycle progression, cytotoxicity and genotoxicity in Chinese hamster ovary (CHO-K1) cells

Author(s)

Franchi, L. P. ^[1] ; De Souza, T. A. J. ^[1] ; Andrioli, W. J. ^{[2, 3]} ; Lima, I. M. S. ^[1] ; Bastos, J. K. ^[3] ; Takahashi, C. S. ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Bloco G. Ave Bandeirantes 3900, BR-14049900 Monte Alegre do Sul, SP - Brazil

^[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ave Bandeirantes 3900, BR-14040900 Vila Monte Alegre, SP - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: WORLD MYCOTOXIN JOURNAL; v. 9, n. 2, p. 237-246, 2016.
• Web of Science Citations: 1

Abstract

Austdiol is a mycotoxin mainly produced by Aspergillus ustus and Mycoleptodiscus indicus. These fungi are found in rye, oats, barley, corn and feed grains; thus, as a potential contaminant of human food and animal feed, this mycotoxin is of great concern. As such, the elucidation of the cytotoxicity and mutagenicity of austdiol is important. In this study, austdiol was purified from a rice-oat solid medium culture of M. indicus using chromatographic separation techniques. Chinese hamster ovary (CHO-K1) cells were then used to study the effect of austdiol on mammalian cell cycle, clonogenicity and DNA damage. Austdiol induced cell cycle arrest in G2/M phase, with a decreased S phase population and increased sub-G1 population. Austdiol also increased the polyploid population. These events resulted in cell death detected 7 days after treatment by clonogenic assay. DNA damage represents the main mechanism of action of austdiol, which induces DNA breaks and increases the frequency of micronuclei and nucleoplasmic bridges in binucleated cells in a CHO-K1 cell line. Moreover, cells exposed to austdiol and doxorubicin (DXR) combined treatments presented a reduced number of colonies and increased frequencies of micronuclei and nucleoplasmic bridges compared with negative control and cells treated with austdiol or DXR alone. (AU)

• FAPESP's process: 10/10863-8 - Cytotoxic and genotoxic effects of nanoparticles in cell lines in vitro.
• Grantee: Leonardo Pereira Franchi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 04/07935-6 - Bioprospection in fungi: the search of lead compounds for drug design and enzymes for pharmaceutical and industrial applications
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants