Acute Phase Response in Buffalo Calves Experimentally Infected with Salmonella typhimurium

Author(s)

Clemente, V. ^[1] ; Santana, A. M. ^[1] ; Silva, D. G. ^[1] ; Silveira, C. R. A. ^[1] ; Pizauro, L. J. L. ^[1] ; Clemente, Z. ^[2] ; Fagliari, J. J. ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Dept Clin & Cirurgia Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] EMBRAPA, Lab Ecotoxicol & Biosseguranca, Rodovia SP-340 Km 127-5, CP 69, BR-13820000 Jaguariuna, SP - Brazil

• Document type: Journal article
• Source: PAKISTAN VETERINARY JOURNAL; v. 36, n. 2, p. 153-158, 2016.
• Web of Science Citations: 2

Abstract

The aim of this study was to evaluate the changes that occur in the acute phase response (APR) of experimentally-induced salmonellosis in buffalo calves and to identify potential indicators of infection. Five buffalo calves received 10 degrees colony-orming units of Salmonella typhimurium, orally. Another five animals were used as control group. Clinical examinations were performed and venous blood was sampled before and throughout 7 days after inoculation. Red blood cell count (RBC), packed cell volume (PCV), hemoglobin (Hb), plasma fibrinogen, serum haptoglobin, ceruloplasmin, transferrin and iron were measured. Severe diarrhea started 72-108 h after inoculation, in all animals. The infectious stimulus induced severe APR, characterized by pyrexia, lymphopenia (4.99 +/- 1.73x10(9) cells/L), increase in fibrinogen (24.7 +/- 4.91 mu mol/L), haptoglobin (21.5 +/- 8.65 mu mol/L) and ceruloplasmin (1370 +/- 374 mg/L), decreased transferrin concentration (3.90 +/- 0.69 g/L) and hypoferremia (15.2 +/- 11.2 mu mol/L). These changes coincided with the onset of clinical signs. Alterations of lower intensity occurred in erythrogram. Based on the magnitude and duration of changes, it is suggested that the combined measurement of serum levels of iron and haptoglobin is a useful tool for identifying newborn buffaloes recently affected by bacterial disease and for monitoring the effectiveness of its treatment. (C) 2015 PVJ. All rights reserved (AU)

• FAPESP's process: 09/12056-5 - Clinical, laboratory and therapeutic study of Salmonella enterica subspecie enterica serovar Typhimurium-induced experimental diarrhea in buffalo calves.
• Grantee: Virna Clemente
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/02415-8 - Clinical, laboratory and therapeutic study of Salmonella enterica subspecie enterica serovar Typhimurium-induced experimental diarrhea in buffalo calves
• Grantee: José Jurandir Fagliari
• Support Opportunities: Regular Research Grants