Red and Infrared Low-Level Laser Therapy Prior to Injury with or without Administration after Injury Modulate Oxidative Stress during the Muscle Repair Process

Author(s)

Ribeiro, Beatriz Guimaraes ^[1] ; Alves, Agnelo Neves ^[1] ; Dias dos Santos, Lucas Andreo ^[2] ; Cantero, Tatiane Matarazzo ^[1] ; Santos Fernandes, Kristianne Porta ^[2] ; Dias, Danielle da Silva ^[1] ; Bernardes, Nathalia ^[3] ; De Angelis, Katia ^{[1, 4]} ; Mesquita-Ferrari, Raquel Agnelli ^{[1, 2]}

Affiliation

^[1] Univ Nove de Julho UNINOVE, Rehabil Dept, Sao Paulo, SP - Brazil

^[2] Univ Nove de Julho UNINOVE, Biophoton Dept, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Hypertens Unit, Heart Inst InCor, Sao Paulo, SP - Brazil

^[4] Univ Nove de Julho UNINOVE, Dept Med, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 11, n. 4 APR 15 2016.
• Web of Science Citations: 14

Abstract

Introduction Muscle injury is common among athletes and amateur practitioners of sports. Following an injury, the production of reactive oxygen species (ROS) occurs, which can harm healthy muscle fibers (secondary damage) and delay the repair process. Low-level laser therapy (LLLT) administered prior to or following an injury has demonstrated positive and protective effects on muscle repair, but the combination of both administration times together has not been clarified. Aim To evaluate the effect of LLLT (660 nm and 780 nm, 10 J/cm(2), 40 mW, 3.2 J) prior to injury with or without the administration after injury on oxidative stress during the muscle repair process. Methods Wistar rats were divided into following groups: control; muscle injury alone; LLLT 660 nm + injury; LLLT 780 nm + injury; LLLT 660 nm before and after injury; and LLLT 780 nm before and after injury. The rats were euthanized on days 1, 3 and 7 following cryoinjury of the tibialis anterior (TA) muscle, which was then removed for analysis. Results Lipid peroxidation decreased in the 660+injury group after one day. Moreover, red and infrared LLLT employed at both administration times induced a decrease in lipid peroxidation after seven days. CAT activity was altered by LLLT in all periods evaluated, with a decrease after one day in the 780+injury+780 group and after seven days in the 780+injury group as well as an increase in the 780+injury and 780+injury+780 groups after three days. Furthermore, increases in GPx and SOD activity were found after seven days in the 780+injury+780 group. Conclusion The administration of red and infrared laser therapy at different times positively modulates the activity of antioxidant enzymes and reduces stress markers during the muscle repair process. (AU)

• FAPESP's process: 14/12381-1 - Comparative analysis of the effect of previous laser therapy associated or not to post treatment on the modulation of inflammatory cytokines, oxidative stress and the determination of muscle fiber type after acute injury
• Grantee: Raquel Agnelli Mesquita Ferrari
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/21540-3 - Comparative effect of low-level laser therapy and low intensity ultrasound on myofiber phenotypes, oxidative stress and proteins during muscle repair
• Grantee: Agnelo Neves Alves
• Support Opportunities: Scholarships in Brazil - Doctorate