Comparative effects of low-level laser therapy pre- and post-injury on mRNA expression of MyoD, myogenin, and IL-6 during the skeletal muscle repair

Author(s)

Alves, Agnelo Neves ^[1] ; Ribeiro, Beatriz Guimares ^[1] ; Santos Fernandes, Kristianne Porta ^[2] ; Costa Souza, Nadhia Helena ^[1] ; Rocha, Lilia Alves ^[3] ; Nunes, Fabio Daumas ^[4] ; Bussadori, Sandra Kalil ^{[1, 2]} ; Mesquita-Ferrari, Raquel Agnelli ^{[1, 2]}

Affiliation

^[1] Univ Nove de Julho UNINOVE, Postgrad Program Rehabil Sci, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[2] Univ Nove de Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, FOUSP, Sch Dent, Dept Mol Pathol, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, FOUSP, Sch Dent, Dept Oral Pathol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 31, n. 4, p. 679-685, MAY 2016.
• Web of Science Citations: 11

Abstract

This study analyzed the effect of pre-injury and post-injury irradiation with low-level laser therapy (LLLT) on the mRNA expression of myogenic regulatory factors and interleukin 6 (IL-6) during the skeletal muscle repair. Male rats were divided into six groups: control group, sham group, LLLT group, injury group; pre-injury LLLT group, and post-injury LLLT group. LLLT was performed with a diode laser (wavelength 780 nm; output power 40 mW' and total energy 3.2 J). Cryoinjury was induced by two applications of a metal probe cooled in liquid nitrogen directly onto the belly of the tibialis anterior (TA) muscle. After euthanasia, the TA muscle was removed for the isolation of total RNA and analysis of MyoD, myogenin, and IL-6 using real-time quantitative PCR. Significant increases were found in the expression of MyoD mRNA at 3 and 7 days as well as the expression of myogenin mRNA at 14 days in the post-injury LLLT group in comparison to injury group. A significant reduction was found in the expression of IL-6 mRNA at 3 and 7 days in the pre-injury LLLT and post-injury LLLT groups. A significant increase in IL-6 mRNA was found at 14 days in the post-injury LLLT group in comparison to the injury group. LLLT administered following muscle injury modulates the mRNA expression of MyoD and myogenin. Moreover, the both forms of LLLT administration were able to modulate the mRNA expression of IL-6 during the muscle repair process. (AU)

• FAPESP's process: 14/12381-1 - Comparative analysis of the effect of previous laser therapy associated or not to post treatment on the modulation of inflammatory cytokines, oxidative stress and the determination of muscle fiber type after acute injury
• Grantee: Raquel Agnelli Mesquita Ferrari
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/21540-3 - Comparative effect of low-level laser therapy and low intensity ultrasound on myofiber phenotypes, oxidative stress and proteins during muscle repair
• Grantee: Agnelo Neves Alves
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/11461-6 - Effects of early treatment with low level laser therapy on the muscle skeletal repair
• Grantee: Beatriz Guimarães Ribeiro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/04452-8 - Photobiomodulation on regulatory myogenic factors expression during skeletal muscle repair following cryolesion in rats
• Grantee: Agnelo Neves Alves
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/17638-2 - Effects of low level laser on regulatory myogenic factors expression and matrix metalloprotease 2 activity during skeletal muscle repair following cryolesion in rats
• Grantee: Raquel Agnelli Mesquita Ferrari
• Support Opportunities: Regular Research Grants