HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC): STATE OF THE ART AND APPLICATIONS

Author(s)

Carla Grazieli Azevedo da Silva ^[1] ; Carla Beatriz Grespan Bottoli ^[2] ; Carol H. Collins ^[3]

Affiliation

^[1] Universidade Estadual de Campinas. Instituto de Química - Brasil

^[2] Universidade Estadual de Campinas. Instituto de Química - Brasil

^[3] Universidade Estadual de Campinas. Instituto de Química - Brasil

• Document type: Journal article
• Source: Química Nova; v. 39, n. 2, p. 210-220, 2016-02-00.

Abstract

Hydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides, etc. Polar compounds are usually poorly retained on reverse-phase liquid chromatography (RP-HPLC) columns or have poor solubility in the apolar mobile phase of normal-phase high performance liquid chromatography (NP-HPLC). Since HILIC uses organic solvents such as ACN or MeOH ( > 70%), also used in RP-HPLC and polar stationary phases similar to NP-HPLC (bare silica, diol, amino, amide, saccharide, zwitterionic stationary phases, etc.), it represents a hybrid of the two separation modes. The high organic content in the MP leads to good compatibility with mass spectrometry (MS), increasing the detectivity. This review describes the fundamentals of HILIC and highlights some interesting applications. (AU)

• FAPESP's process: 13/25933-0 - Preparation of silica and mixed oxides monoliths for use in capillary liquid chromatography
• Grantee: Carla Beatriz Grespan Bottoli
• Support Opportunities: Regular Research Grants