Comparative analysis of expression profiling of the trypsin and chymotrypsin genes from Lepidoptera species with different levels of sensitivity to soybean peptidase inhibitors

Author(s)

Souza, Thais P. ^[1] ; Dias, Renata O. ^[1] ; Castelhano, Elaine C. ^[1] ; Brandao, Marcelo M. ^[2] ; Moura, Daniel S. ^[3] ; Silva-Filho, Marcio C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 11, BR-13400918 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Ave Candido Rondon 400, BR-13083875 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, Ave Padua Dias 11, BR-13400918 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 196, p. 67-73, JUN-JUL 2016.
• Web of Science Citations: 4

Abstract

Peptidase inhibitors (Pis) are essential proteins involved in plant resistance to herbivorous insects, yet many insect species are able to escape the negative effects of these molecules. We compared the effects of acute and chronic ingestion of soybean peptidase inhibitors (SPIs) on Spodoptera frugiperda and Diatraea saccharalis, two Lepidoptera species with different sensitivities to SPI ingestion. We analyzed the trypsin and chymotrypsin gene expression profiles in both species. Acute exposure of S. frugiperda to the inhibitors activated seven genes (SfChy5, SfChy9, SfChy19, SfChy22, SfTry6, SfTry8, and SfTry10), whereas chronic exposure activated 16 genes (SfChy2, SfChy4, SfChy5, SfChy8, SfChy9, SfChy11, SfChy12, SfChy15, SfChy17, SfChy21, SfChy22, SfTry6, SfTry8, SfTry9, SfTry10, and SfTry12). By contrast, the challenge of D. saccharalis with SPIs did not differentially induce the expression of trypsin- or chymotrypsin-encoding genes, with the exception of DsChy7. Bayesian phylogenetic analysis of S. frugiperda trypsin protein sequences revealed two gene clades: one composed of genes responsive to the SPIs and a second composed of the unresponsive genes. D. saccharalis trypsin proteins were clustered nearest to the S. frugiperda unresponsive genes. Overall, our findings support a hypothesized mechanism of resistance of Noctuidae moths to SPIs, involving gene number expansion of trypsin and chymotrypsin families and regulation of gene expression, which could also explain the variable susceptibility between S. frugiperda and D. saccharalis to these plant inhibitors. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 11/00417-3 - System biology techniques applied to the agriculture: transcriptomes and interactomes analyses
• Grantee: Marcelo Mendes Brandao
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 12/03040-0 - Evolution and characterization of serine protease gene family and its association with the feeding behavior in Noctuidae moths
• Grantee: Renata de Oliveira Dias
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 08/52067-3 - Herbivory and intracellular transport of proteins
• Grantee: Márcio de Castro Silva Filho
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 09/15421-6 - Deciphering the Spodoptera frugiperda adaptative mechanisms to plant proteinase inhibitors
• Grantee: Thais Paula de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/17110-5 - Characterization of molecular bases involved in the polifagism of herbivore insect
• Grantee: Renata de Oliveira Dias
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 08/57908-6 - National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants