Enzyme-assisted extraction of phenolics from winemaking by-products: Antioxidant potential and inhibition of alpha-glucosidase and lipase activities

Author(s)

de Camargo, Adriano Costa ^{[1, 2]} ; Bismara Regitano-d'Arce, Marisa Aparecida ^[2] ; Telles Biasoto, Aline Camarao ^[3] ; Shahidi, Fereidoon ^[1]

Affiliation

^[1] Mem Univ Newfoundland, Dept Biochem, St John, NF A1B 3X9 - Canada

^[2] Univ Sao Paulo, Dept Agri Food Ind Food & Nutr, Luiz de Queiroz Coll Agr, Ave Padua Dias 11, POB 9, BR-13418900 Piracicaba, SP - Brazil

^[3] Embrapa Semiarido, Rodovia BR 428, Km 152, POB 23, BR-56302970 Petrolina, PE - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 212, p. 395-402, DEC 1 2016.
• Web of Science Citations: 51

Abstract

Phenolics in food and agricultural processing by-products exist in the soluble and insoluble-bound forms. The ability of selected enzymes in improving the extraction of insoluble-bound phenolics from the starting material (experiment I) or the residues containing insoluble-bound phenolics (experiment II) were evaluated. Pronase and Viscozyme improved the extraction of insoluble-bound phenolics as evaluated by total phenolic content, antioxidant potential as determined by ABTS and DPPH assays, and hydroxyl radical scavenging capacity, reducing power as well as evaluation of inhibition of alpha-glucosidase and lipase activities. Viscozyme released higher amounts of gallic acid, catechin, and prodelphinidin dimer A compared to Pronase treatment. Furthermore, p-coumaric and caffeic acids, as well as procyanidin dimer B, were extracted with Viscozyme but not with Pronase treatment. Solubility plays an important role in the bioavailability of phenolic compounds, hence this study may assist in better exploitation of phenolics from winemaking by-products as functional food ingredients and/or supplements. (C) 2016 Published by Elsevier Ltd. (AU)

• FAPESP's process: 12/17683-0 - GAMMA RADIATION AS A TOOL FOR AGRO-INDUSTRIAL WASTES MICROBIOLOGICAL SAFETY AND ITS EFFECT ON THE ANTIOXIDANT PROPERTIES EVALUATED BY IN VITRO TESTS AND MODEL SYSTEMS
• Grantee: Adriano Costa de Camargo
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/00336-4 - Enzyme assited extraction of insoluble-bound phenolics from grape by-products: antioxidant and bioactivity potential
• Grantee: Adriano Costa de Camargo
• Support type: Scholarships abroad - Research Internship - Doctorate