Enzyme assited extraction of insoluble-bound phenolics from grape by-products: antioxidant and bioactivity potential

• Grant number: 15/00336-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): June 01, 2015
• Effective date (End): December 31, 2015
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Marisa Aparecida Bismara Regitano d'Arce
• Grantee: Adriano Costa de Camargo
• Supervisor: Fereidoon Shahidi
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Research place: Memorial University of Newfoundland (MUN), Canada
• Associated to the scholarship: 12/17683-0 - GAMMA RADIATION AS A TOOL FOR AGRO-INDUSTRIAL WASTES MICROBIOLOGICAL SAFETY AND ITS EFFECT ON THE ANTIOXIDANT PROPERTIES EVALUATED BY IN VITRO TESTS AND MODEL SYSTEMS, BP.DR

Abstract

Food phenolics are found in the soluble fractions (free and esterified forms) as well as in the insoluble-bound form. We recently demonstrated that, despite the process (juice or winemaking), insoluble-bound phenolics are the major fraction in grape processing by-products (BRS Cora, Isabel, Tempranillo, and Syrah varieties). Furthermore, in another work, we also demonstrated that gamma-irradiation increased the content of insoluble-bound phenolics, probably due to gamma-irradiation induced cross-linking. Insoluble-bound phenolics from grapes inhibited in vitro peroxyl radical induced human LDL-cholesterol oxidation and copper induced DNA strand breakage. However, it has been reported that absorption of insoluble-bound phenolics is limited. Thus, in the present work, enzyme assited extraction will be studied in order to release insoluble-bound phenolics which may be further used as functional food ingredients and/or supplements manufacture. Besides improving the extraction, the process may also lead to chemical structure changes which will likely affect the antioxidant activity of these by-products. Thus, the enzyme assisted extraction will be evaluated for its effect on phenolic profile using HPLC-DAD-ESI-MSn, antioxidante assays and in deactivating seletected enzymes with eventual health benefits. The present study will shed further light on the importance as well as potential use of insoluble-bound phenolics of food plants. Additionaly, the enzyme assisted release of insoluble-bound phenolics may minimize the effect of food processing, such as gamma-irradiation, in inducing insoluble-bound phenolics generation via cross-linking. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CAMARGO, ADRIANO COSTA; BISMARA REGITANO-D'ARCE, MARISA APARECIDA; TELLES BIASOTO, ALINE CAMARAO; SHAHIDI, FEREIDOON. Enzyme-assisted extraction of phenolics from winemaking by-products: Antioxidant potential and inhibition of alpha-glucosidase and lipase activities. Food Chemistry, v. 212, p. 395-402, DEC 1 2016. (12/17683-0, 15/00336-4)

DE CAMARGO, ADRIANO COSTA; BISMARA REGITANO-D'ARCE, MARISA APARECIDA; SHAHIDI, FEREIDOON. Phenolic Profile of Peanut By-products: Antioxidant Potential and Inhibition of Alpha-Glucosidase and Lipase Activities. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v. 94, n. 7, p. 959-971, JUL 2017. (12/17683-0, 15/00336-4)

BUENO-BORGES, LARISSA BRAGA; DE CAMARGO, ADRIANO COSTA; SANGALETTI-GERHARD, NAIANE; PEREIRA DOS SANTOS, GRASIELA CRISTINA; DE ALENCAR, SEVERINO MATIAS; SHAHIDI, FEREIDOON; BISMARA REGITANO-D'ARCE, MARISA APARECIDA. A Highly Stable Soybean Oil-Rich Miscella Obtained by Ethanolic Extraction as a Promising Biodiesel Feedstock. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v. 94, n. 8, p. 1101-1109, AUG 2017. (12/17683-0, 15/00336-4, 16/01956-9, 13/03650-6)

DE CAMARGO, ADRIANO COSTA; BISMARA REGITANO-D'ARCE, MARISA APARECIDA; RASERA, GABRIELA BOSCARIOL; CANNIATTI-BRAZACA, SOLANGE GUIDOLIN; DO PRADO-SILVA, LEONARDO; ALVARENGA, VERONICA ORTIZ; SANT'ANA, ANDERSON S.; SHAHIDI, FEREIDOON. Phenolic acids and flavonoids of peanut by-products: Antioxidant capacity and antimicrobial effects. Food Chemistry, v. 237, p. 538-544, DEC 15 2017. (12/17683-0, 15/00336-4)

DE CAMARGO, ADRIANO COSTA; BISMARA REGITANO-D'ARCE, MARISA APARECIDA; DE ALENCAR, SEVERINO MATIAS; CANNIATTI-BRAZACA, SOLANGE GUIDOLIN; FERREIRA DE SOUZA VIEIRA, THAIS MARIA; SHAHIDI, FEREIDOON. Chemical Changes and Oxidative Stability of Peanuts as Affected by the Dry-Blanching. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v. 93, n. 8, p. 1101-1109, AUG 2016. (12/17683-0, 15/00336-4)

DE OLIVEIRA, WALKIA POLLIANA; TELLES BIASOTO, ALINE CAMARAO; MARQUES, VALQUIRIA FERNANDA; DOS SANTOS, IEDA MARIA; MAGALHAES, KEDMA; CORREA, LUIZ CLAUDIO; NEGRO-DELLACQUA, MELISSA; MIRANDA, MARIA SPINOLA; DE CAMARGO, ADRIANO COSTA; SHAHIDI, FEREIDOON. Phenolics from Winemaking By-Products Better Decrease VLDL-Cholesterol and Triacylglycerol Levels than Those of Red Wine in Wistar Rats. Journal of Food Science, v. 82, n. 10, p. 2432-2437, OCT 2017. (12/17683-0, 15/00336-4)

SHAHIDI, FEREIDOON; DE CAMARGO, ADRIANO COSTA. Tocopherols and Tocotrienols in Common and Emerging Dietary Sources: Occurrence, Applications, and Health Benefits. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 17, n. 10, OCT 2016. (12/17683-0, 15/00336-4)

DE CAMARGO, ADRIANO COSTA; SCHWEMBER, ANDRES R.; PARADA, ROBERTO; GARCIA, SANDRA; MAROSTICA JUNIOR, MARIO ROBERTO; FRANCHIN, MARCELO; BISMARA REGITANO-D'ARCE, MARISA APARECIDA; SHAHIDI, FEREIDOON. Opinion on the Hurdles and Potential Health Benefits in Value-Added Use of Plant Food Processing By-Products as Sources of Phenolic Compounds. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 19, n. 11, NOV 2018. (12/17683-0, 15/50333-1, 16/15563-9, 15/00336-4)