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Development of microparticles containing grape skin extract through the emerging technique SAS and evaluation of their antioxidant and anti-inflammatory effects in the treatment of induced Colitis in rats

Grant number: 17/23657-6
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): August 01, 2018
Effective date (End): October 31, 2022
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal Investigator:Mário Roberto Maróstica Junior
Grantee:Ana Paula da Fonseca Machado
Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):19/12372-6 - Polyphenols encapsulation obtained from the grape residue by supercritical antisolvent technique: powder characterization, chemical stability and controlled release study in simulated gastrointestinal fluids, BE.EP.PD


The vegetal residue generated by the wine industry has been recognized as an excellent source of bioactive compounds due to its expressive amount of polyphenols, which have been shown to have protective effects against several chronic diseases, such as Cancer, cardiovascular diseases and inflammatory diseases, given by attenuation of oxidative stress and blocking of oxidative signaling pathways. However, sometimes the efficiency of the phenolic compounds is limited due to their poor solubility in aqueous medium and the low stability under severe conditions of acidity and alkalinity, and high temperatures, besides the low availability after oral administration. Starting from the mouth, the polyphenols begin their journey through the gastrointestinal tract, where they are submitted to several physical-chemical-biological transformations, of which they can lose their bioactivities completely. In order to overcome these problems, the development of polyphenol encapsulation systems (such as anthocyanins) through non-thermal, environmentally and humanly safe techniques are interesting to be employed in order to increase and improve the rate of release and its bioavailability to the target regions, especially when this regions is the lower gastrointestinal tract. In this context, this project proposes to investigate the antioxidant and anti-inflammatory properties of microparticles rich in grape skin extract produced by the emerging technique Supercritical Antisolvent (SAS) in the treatment of colitis, which is a type of inflammation of the large intestine, induced by acid 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats. In order to achieve this objective, the project was strategically structured in five steps: i) receigt, pre-treatment and characterization of the raw material (grape skin); ii) extraction and characterization of the extract; iii) extract encapsulation by the SAS technique and characterization of the microparticles; iv) administration of microparticles and unencapsulated dry extract in rats with induced Colitis; and v) analyzes of blood, tissues and faeces of animals. By performing these steps, it is expected that the microparticles produced by the SAS technique have great functional potential in vivo, thus contributing to the promotion of health and human well-being. (AU)

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Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, CAMILA TELLES; DA SOUZA, MARIANA CORREA; DA FONSECA MACHADO, ANA PAULA; DO NASCIMENTO, ROBERTO DE PAULA; DA CUNHA, DIOGO THIMOTEO; NEVES BEZERRA, ROSANGELA MARIA; ROSTAGNO, MAURICIO ARIEL. Thermal stability and sensory evaluation of a bioactive extract from roasted coffee (Coffea arabica) beans added at increasing concentrations to conventional bread. JOURNAL OF FOOD PROCESSING AND PRESERVATION, v. 45, n. 11, . (18/14582-5, 16/19930-6, 17/23657-6, 13/04304-4, 18/17089-8)
MACHADO, ANA PAULA DA FONSECA; DO NASCIMENTO, ROBERTO DE PAULA; ALVES, MARIANA DA ROCHA; REGUENGO, LIVIA MATEUS; MAROSTICA JUNIOR, MARIO ROBERTO. razilian tucuma-do-Amazonas (Astrocaryum aculeatum) and tucuma-do-Para (Astrocaryum vulgare) fruits: bioactive composition, health benefits, and technological potentia. Food Research International, v. 151, . (18/11069-5, 19/25048-2, 19/03228-9, 17/23657-6, 15/50333-1, 15/13320-9)
DE PAULA DO NASCIMENTO, ROBERTO; DA FONSECA MACHADO, ANA PAULA; LIMA, VERENA SILVA; TOMAZINI MUNHOZ MOYA, AMANDA MARIA; REGUENGO, LIVIA MATEUS; BOGUSZ JUNIOR, STANISLAU; LEAL, RAQUEL FRANCO; CAO-NGOC, PHU; ROSSI, JEAN CHRISTOPHE; LECLERCQ, LAURENT; et al. Chemoprevention with a tea from hawthorn (Crataegus oxyacantha) leaves and flowers attenuates colitis in rats by reducing inflammation and oxidative stress. FOOD CHEMISTRY-X, v. 12, . (15/50333-1, 15/13320-9, 18/11069-5, 17/23657-6)
MACHADO, ANA PAULA DA FONSECA; GERALDI, MARINA VILAR; NASCIMENTO, ROBERTO DE PAULA DO; MOYA, AMANDA MARIA TOMAZINI MUNHOZ; VEZZA, TERESA; DIEZ-ECHAVE, PATRICIA; JUAN GALVAZ, JULIO; CAZARIN, CINTHIA BAU BETIM; MAROSTICA JUNIOR, MARIO ROBERTO. Polyphenols from food by-products: An alternative or complementary therapy to IBD conventional treatments. Food Research International, v. 140, . (18/11069-5, 19/12244-8, 15/50333-1, 17/23657-6)
REGUENGO, LIVIA MATEUS; DO NASCIMENTO, ROBERTO DE PAULA; MACHADO, ANA PAULA DA FONSECA; MAROSTICA JUNIOR, MARIO ROBERTO. Signaling pathways and the potential anticarcinogenic effect of native Brazilian fruits on breast cancer. Food Research International, v. 155, p. 13-pg., . (15/13320-9, 18/11069-5, 19/03228-9, 17/23657-6, 15/50333-1, 19/25048-2, 19/12244-8)
DO NASCIMENTO, ROBERTO DE PAULA; REGUENGO, LIVIA MATEUS; DA FONSECA MACHADO, ANA PAULA; MAROSTICA JUNIOR, MARIO ROBERTO. he preventive and therapeutic potential of native Brazilian fruits on colorectal cance. FOOD BIOSCIENCE, v. 46, . (18/11069-5, 19/03228-9, 17/23657-6, 15/50333-1, 19/25048-2, 15/13320-9)
MACHADO, ANA PAULA DA FONSECA; MONTES, ANTONIO; VALOR, DIEGO. Co-precipitation of grape residue extract using sub- and supercritical CO2 technology. JOURNAL OF CO2 UTILIZATION, v. 61, p. 14-pg., . (19/12372-6, 17/23657-6)
DO NASCIMENTO, ROBERTO DE PAULA; RIZZATO, JULIA SOTO; POLEZI, GABRIELE; MOYA, AMANDA MARIA TOMAZINI MUNHOZ; SILVA, MILENA FERREIRA; MACHADO, ANA PAULA DA FONSECA; FRANCHI JUNIOR, GILBERTO CARLOS; BORGUINI, RENATA GALHARDO; SANTIAGO, MANUELA CRISTINA PESSANHA DE ARAUJO; PAIOTTI, ANA PAULA RIBEIRO; et al. Freeze-dried jaboticaba (Myrciaria jaboticaba) peel powder, a rich source of anthocyanins and phenolic acids, mitigates inflammation-driven colorectal cancer in mice. FOOD BIOSCIENCE, v. 53, p. 10-pg., . (15/13320-9, 20/00414-3, 19/03228-9, 15/50333-1, 17/23657-6, 18/11069-5)
MACHADO, ANA PAULA DA FONSECA; ALVES, MARIANA DA ROCHA; NASCIMENTO, ROBERTO DE PAULA DO; REGUENGO, LIVIA MATEUS; MAROSTICA JR, MARIO ROBERTO. Antiproliferative effects and main molecular mechanisms of Brazilian native fruits and their by-products on lung cancer. Food Research International, v. 162, p. 9-pg., . (17/23657-6, 19/03228-9, 15/50333-1, 19/12244-8, 15/13320-9, 18/11069-5)
DE PAULA DO NASCIMENTO, ROBERTO; DA FONSECA MACHADO, ANA PAULA; GALVEZ, JULIO; BETIM CAZARIN, CINTHIA BAU; MAROSTICA JUNIOR, MARIO ROBERTO. Ulcerative colitis: Gut microbiota, immunopathogenesis and application of natural products in animal models. Life Sciences, v. 258, p. 22-pg., . (17/23657-6, 15/13320-9, 18/11069-5, 15/50333-1)
DO NASCIMENTO, ROBERTO DE PAULA; MOYA, AMANDA MARIA TOMAZINI MUNHOZ; MACHADO, ANA PAULA DA FONSECA; GERALDI, MARINA VILAR; DIEZ-ECHAVE, PATRICIA; VEZZA, TERESA; GALVEZ, JULIO; CAZARIN, CINTHIA BAIL BETIM; MAROSTICA, JR., MARIO ROBERTO. Review on the potential application of non-phenolic compounds from native Latin American food byproducts in inflammatory bowel diseases. Food Research International, v. 139, . (15/50333-1, 15/13320-9, 19/12244-8, 17/23657-6, 18/11069-5)

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