Effect of photobiomodulation on endothelial cell exposed to Bothrops jararaca venom

Author(s)

Barufi Franco, Ana Tereza ^[1] ; Goncalves Silva, Luciana Miato ^[1] ; Costa, Marcilia Silva ^[2] ; Zamuner, Silvia Fernanda ^[1] ; Vieira, Rodolfo Paula ^[1] ; Pereira Teixeira, Catarina de Fatima ^[3] ; Zamuner, Stella Regina ^{[1, 4]}

Affiliation

^[1] Univ Nove Julho UNINOVE, R Vergueiro 235, BR-01504001 Sao Paulo, SP - Brazil

^[2] Univ Vale do Paraiba, Inst Res & Dev, Sao Paulo - Brazil

^[3] Inst Butantan, Lab Farmacol, Sao Paulo - Brazil

^[4] Rua Vergueiro, 234 Bairro Liberdade, BR-01504000 Sao Paulo - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 31, n. 5, p. 1017-1025, JUL 2016.
• Web of Science Citations: 2

Abstract

Bleeding is a common feature in envenoming caused by Bothrops snake venom due to extensive damage to capillaries and venules, producing alterations in capillary endothelial cell morphology. It has been demonstrated, in vivo, that photobiomodulation (PBM) decreases hemorrhage after venom inoculation; however, the mechanism is unknown. Thus, the objective was to investigate the effects of PBM on a murine endothelial cell line (tEnd) exposed to Bothrops jararaca venom (BjV). Cells were exposed to BjV and irradiated once with either 660-or 780-nm wavelength laser light at energy densities of 4 and 5 J/cm(2), respectively, and irradiation time of 10 s. Cell integrity was analyzed by crystal violet and cell viability/mitochondrial metabolism by MTT assay. The release of lactic dehydrogenase (LDH) was quantified as a measure of cell damage. In addition, cytokine IL1-beta levels were measured in the supernatant. PBM at 660 and 780 nm wavelength was able to increase cellular viability and decrease the release of LDH and the loss of cellular integrity. In addition, the concentration of pro-inflammatory cytokine IL1-beta was reduced after PBM by both wavelengths. The data reported herein indicates that irradiation with red or near-infrared laser resulted in protection on endothelial cells after exposure to Bothrops venom and could be, at least in part, a reasonable explanation by the beneficial effects of PBM inhibiting the local effects induced by Bothrops venoms, in vivo. (AU)

• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants