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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Virulence-associated genes, antimicrobial resistance and molecular typing of Salmonella Typhimurium strains isolated from swine from 2000 to 2012 in Brazil

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Author(s):
Almeida, F. [1] ; Medeiros, M. I. C. [2] ; Kich, J. D. [3] ; Falcao, J. P. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Inst Adolfo Lutz Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Embrapa Suinos & Aves, Concordia, SC - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Applied Microbiology; v. 120, n. 6, p. 1677-1690, JUN 2016.
Web of Science Citations: 16
Abstract

Aims: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. Methods and Results: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 77.8% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. Conclusions: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. Significance and Impact of the Study: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity of S. Typhimurium isolates from swine origin in Brazil, the fourth largest producer of pigs worldwide. (AU)

FAPESP's process: 12/19132-1 - Molecular characterization of Salmonella Typhimurium strains isolated from humans, food and animals in the Southeast and South regions of Brazil
Grantee:Juliana Pfrimer Falcão
Support type: Regular Research Grants