Dendritic Cells Are Involved in the Effects of Exercise in a Model of Asthma

Author(s):Show less -

Mackenzie, Breanne ^[1] ; Andrade-Sousa, Adilson Santos ^[1] ; Oliveira-Junior, Manoel Carneiro ^[1] ; Assumpcao-Neto, Erasmo ^[1] ; Rodrigues Brandao-Rangel, Maysa Alves ^[1] ; Silva-Renno, Adriano ^[1] ; Santos-Dias, Alana ^[1] ; Cicko, Sanja ^{[2, 3]} ; Grimm, Melanie ^{[2, 3]} ; Mueller, Tobias ^{[2, 3]} ; Ligeiro Oliveira, Ana Paula ^[1] ; Martins, Milton Arruda ^[4] ; Idzko, Marco ^{[2, 3]} ; Vieira, Rodolfo Paula ^[1]

Affiliation

^[1] Nove de Julho Univ UNINOVE, Lab Pulm & Exercise Immunol LABPEI, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[2] Univ Hosp Freigurg, COPD, Freiburg - Germany

^[3] Univ Hosp Freigurg, Asthma Res Grp, Freiburg - Germany

^[4] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 20, Sao Paulo - Brazil

• Document type: Journal article
• Source: MEDICINE AND SCIENCE IN SPORTS AND EXERCISE; v. 48, n. 8, p. 1459-1467, AUG 2016.
• Web of Science Citations: 9

Abstract

Introduction: This study investigated the effects of aerobic exercise (AE) on both the maturation of dendritic cells (DC) and the activation of lymphocytes in a mouse model of chronic allergic airway inflammation. Methods: C57BL/6 mice distributed into control, exercise, ovalbumin (OVA), and OVA + exercise groups were submitted to OVA sensitization and challenge. Treadmill training was performed for 4 wk, and mice were assessed for classical features of chronic allergic airway inflammation as well as dendritic cell activation and T-lymphocyte response. Results: AE reduced OVA-induced eosinophilic inflammation as observed in bronchoalveolar lavage fluid (P < 0.001), airway walls (P < 0001), and also reduced collagen deposition (P < 0.001). AE also reduced bronchoalveolar lavage fluid cytokines (interleukin {[}IL]-4, P < 0.001; IL-5, P < 0.01; IL-6, P < 0.001; IL-13, P < 0.01; and tumor necrosis factor >, P < 0.01). Cells derived from mediastinal lymphnodes of AE animals that were restimulated with OVA produced less IL-4 (P < 0.01), IL-5 (P < 0.01), and IL-13 (P < 0.001). In addition, AE reduced both DC activation, as demonstrated by reduced release of IL-6 (P < 0.001), CXCL1/KC (P < 0.01), IL-12p70 (P < 0.01), and tumor necrosis factor > (P < 0.05) and DC maturation, as demonstrated by lower MCH-II expression (P < 0.001). Conclusion: AE attenuated dendritic cell and lymphocyte activation and maturation, which contributed to reduced airway inflammation and remodeling in the OVA model of chronic allergic airway inflammation. (AU)

• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 15/50079-8 - A deep dive in the world of dendritic cells: their role in asthma and in exercise immunology
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Regular Research Grants