Improved catalytic properties of Candida antarctica lipase B multi-attached on tailor-made hydrophobic silica containing octyl and multifunctional amino- glutaraldehyde spacer arms

Author(s)

Vescovi, Vinicius ; Kopp, Willian ; Guisan, Jose M. ; Giordano, Raquel L. C. ; Mendes, Adriano A. ; Tardioli, Paulo W.

• Document type: Journal article
• Source: Process Biochemistry; v. 51, n. 12, p. 2055-2066, DEC 2016.
• Web of Science Citations: 20

Abstract

This work evaluated the immobilization of Candida antarctica lipase B (CALB) on a new heterofunctional support, prepared by silanization of silica particles with triethoxy(octyl)silane and trietoxy(3-aminopropyl)silane, followed by activation with glutaraldehyde. The new support (octyl-silica-amino-glutaraldehyde, OSGlu) exhibits features of an anionic exchanger and hydrophobic adsorbent capable of adsorbing and covalently linking the lipase under mild conditions in a single step. As comparison, CALB was also immobilized on silica coated with octyl, amino-glutaraldehyde, octyl and glyoxyl, and octyl and epoxy groups. Among these supports, CALB immobilized on OSGlu (namely CALB-OSGlu), showed the highest recovered activity (71 +/- 2%) and thermal stability in tert-butyl alcohol (full activity was recovered after 100 h at 65 degrees C). CALK immobilized on silica coated with octyl or amino-glutaraldehyde yielded biocatalysts with lower recovered activities, compared to CALB-OSGlu, with values of 43% and 32%, respectively. CALB-OSGlu was chosen to be evaluated in the synthesis of fructose oleate in tert-butyl alcohol medium and at high temperature, achieving conversions above 70% in nine 6 h-cycles at 55 degrees C. CALB-OSGlu showed better operational stability than benchmark immobilized CALB.(Novozym 435) in the same reaction conditions, with the conversion of the later decreasing from 84% (first cycle) to 53% (ninth cycle). (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 11/23194-0 - Enzymatic synthesis of fatty esters using heating assisted by microwave
• Grantee: Paulo Waldir Tardioli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/12980-5 - ECCE 10 + ECAB 3 + EPIC 5
• Grantee: Paulo Waldir Tardioli
• Support Opportunities: Research Grants - Meeting - Abroad