| Full text | |
| Author(s): |
Monroy, Yaneth M.
;
Rodrigues, Rodney A. F.
;
Sartoratto, Adilson
;
Cabral, Fernando A.
Total Authors: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF SUPERCRITICAL FLUIDS; v. 118, p. 11-18, DEC 2016. |
| Web of Science Citations: | 19 |
| Abstract | |
Supercritical carbon dioxide (scCO(2)), ethanol (EtOH), and water are considered GRAS (Generally Recognized as Safe) solvents. They exhibit considerable advantages in the extraction of phenolic compounds with a wide range of polarities, such as the anthocyanins found in purple corn (Zea mays L.). To evaluate the effect of ethanol and water as co-solvents in the scCO(2) extraction of phenolic compounds of purple corn cobs, extracts were obtained at 400 bar and 50 degrees C using EtOH (CE), water (CW), and mixtures of EtOH/water 50% (CE50), and EtOH/water 70% (CE70) as co-solvents of scCO(2). The overall yield (X-0), the extraction yield of total monomeric anthocyanins (TMA), total phenolics (TP), total flavonoids (TF) were determined in the extracts, as well as the antioxidant activity (AA) expressed as EC50/DPPH, color, and presence of specific anthocyanins by HPLC. The lowest extraction yield (4.15%) was obtained when ethanol was used as co-solvent (CE), and the highest yield (24.4%) was observed when using a mixture of EtOH/water (70%, CE70) as co-solvent. The presence of EtOH and water was essential to obtain concentrated phenolic extracts, with values around 66 mg cyanidin-3-glycoside equivalent per gram of dried extract, 290 mg gallic acid equivalent per gram, and 117 mg catechin equivalent per gram, and antioxidant activity of CE50 =31 mu g/mL by DPPH. (C) 2016 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 12/51317-1 - Development of products and processes with supercritical technology: construction of one multipurpose unit to obtain extracts of industrial interest. |
| Grantee: | Fernando Antonio Cabral |
| Support Opportunities: | Regular Research Grants |