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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Toxicity analysis of ocular prosthesis acrylic resin with or without pigment incorporation in human conjunctival cell line

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Author(s):
Freitas da Silva, Emily Vivianne ; Goiato, Marcelo Coelho ; Bonatto, Liliane da Rocha ; de Medeiros, Rodrigo Antonio ; dos Santos, Daniela Micheline ; Rangel, Elidiane Cipriano ; Penha de Oliveira, Sandra Helena
Total Authors: 7
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 36, p. 180-185, OCT 2016.
Web of Science Citations: 1
Abstract

The aim of this study was to evaluate the influence of pigment incorporation on the cytotoxicity of ocular prosthesis N1 color acrylic resin. Nine samples were manufactured by, heat-polymerization in water bath and divided into 3 groups: acrylic resin without pigment incorporation (group R), acrylic resin with pigment incorporation (group RP), and acrylic pigment (group P). Eluates formed after 72 h of sample immersion in medium were incubated with conjunctival cell line (Chang conjunctival cells) for 72 h. The negative control group consisted in medium without samples (group C). The cytotoxic effect from the eluates was evaluated using MTT assay (cell proliferation), ELISA assay (quantification of IV, IL beta, TNF alpha and CCL3/MIP1 alpha) and RT-PCR assay (mRNA expression of COL IV, TGF beta and MMP9). Data were submitted to ANOVA with Bonferroni post-tests (p < 0.05). All groups were considered non-cytotoxic based on cell proliferation. However, resin with pigment incorporation showed significant IL6 quantity increase. Resin without pigment incorporation exhibited higher mRNA expression of COL IV, MMP9 and TGF beta, however it was also observed for the negative control group. The materials exhibited divergent biological behavior. Despite the pigment incorporation that resulted in an increase of IL6, no cytotoxicity was observed based on cell proliferation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/11830-4 - In vitro analysis of cell proliferation and inflammatory mediators production from conjunctival cells that are stimulated by ocular prostheses components
Grantee:Emily Vivianne Freitas da Silva
Support Opportunities: Scholarships in Brazil - Master