The L-amino acid oxidase from Calloselasma rhodostoma snake venom modulates apoptomiRs expression in Bcr-Abl-positive cell lines

Author(s)

Burin, Sandra Mara ; Berzoti-Coelho, Maria Gabriela ; Cominal, Jucara Gastaldi ; Ambrosio, Luciana ; Torqueti, Maria Regina ; Sampaio, Suely Vilela ; de Castro, Fabiola Attie

• Document type: Journal article
• Source: Toxicon; v. 120, p. 9-14, SEP 15 2016.
• Web of Science Citations: 10

Abstract

Anti-apoptotic genes and apoptomiRs deregulated expression contribute to apoptosis resistance in chronic myeloid leukemia (CML) Bcr-Abl(+) cells. Here, the L-amino acid oxidase from Calloselasma rhodostoma (CR-LAAO) venom altered the apoptotic machinery regulation by modulating the expression of the miR-145, miR-26a, miR-142-3p, miR-21, miR-130a, and miR-146a, and of the apoptosis-related proteins Bid, Bim, Bcl-2, Ciap-2, c-Flip, and Mcl-1 in Bcr-Abl(+) cells. CR-LAAO is a potential tool to instigate apoptomiRs regulation that contributes to drive CML therapy. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 15/23555-3 - Regulators microRNAs of HIPPO pathway in Chronic Myeloid Leukemia
• Grantee: Maria Gabriela Berzoti Coelho
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/25637-7 - Epigenetic modulation of apoptotic machinery in Bcr-Abl positive cells by BmooLAAO-I and MjTX-I toxins
• Grantee: Sandra Mara Burin de Menezes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
• Grantee: Suely Vilela
• Support Opportunities: Research Projects - Thematic Grants