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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quality of sweat test (ST) based on the proportion of sweat sodium (Na) and sweat chloride (Cl) as diagnostic parameter of cystic fibrosis: are we on the right way?

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Faria, Alethea Guimaraes ; Lima Marson, Fernando Augusto ; de Souza Gomez, Carla Cristina ; Goncalves de Oliveira Ribeiro, Maria Angela ; Morais, Lucas Brioschi ; Servidoni, Maria de Fatima ; Bertuzzo, Carmen Silvia ; Sakano, Eulalia ; Goto, Maura ; Paschoal, Ilma Aparecida ; Pereira, Monica Corso ; Hessel, Gabriel ; Levy, Carlos Emilio ; Dalbo Contrera Toro, Adyleia Aparecida ; Peixoto, Andressa Oliveira ; Ribeiro Simoes, Maria Cristina ; Lomazi, Elizete Aparecida ; Negrao Nogueira, Roberto Jose ; Ribeiro, Antonio Fernando ; Ribeiro, Jose Dirceu
Total Authors: 20
Document type: Journal article
Source: DIAGNOSTIC PATHOLOGY; v. 11, OCT 26 2016.
Web of Science Citations: 3
Abstract

Background: To assess the quality of sweat test (ST) based on the proportion of sweat sodium and sweat chloride as diagnostic parameter of cystic fibrosis (CF). Methods: A retrospective study of 5,721 sweat samples and subsequent descriptive analysis were carried out. The test was considered ``of good quality{''} (correct) when: (i) sweat chloride was lower than 60 mEq/L, and sweat sodium was higher than sweat chloride; (ii) sweat chloride was higher than 60 mEq/L, and sweat sodium was lower than sweat chloride. Results: The study included 5,692/5,721 sweat samples of ST which had been requested due to clinical presentations compatible with CF and/or neonatal screenings with altered immunoreactive trypsinogen values. Considering the proportion of sweat sodium and sweat chloride as ST quality parameter, the test was performed correctly in 5,023/5,692 (88.2 %) sweat samples. The sweat chloride test results were grouped into four reference ranges for chloride (i) chloride >= 30 mEq/L: 3,651/5,692 (64.1 %); (ii) chloride < 30 mEq/L to < 40 mEq/L: 652/5,692 (11.5 %); (iii) >= 40 mEq/L to < 60 mEq/L: 673/5,692 (11.8 %); (iv) >= 60 mEq/L: 716/5,692 (12.6 %). In the comparative analysis, there was no association between ST quality and: (i) symptoms to indicate a ST {[}respiratory (p = 0.084), digestive (p = 0.753), nutritional (p = 0.824), and others (p = 0.136)], (ii) sweat weight (p = 0.416). However, there was a positive association with: (i) gender, (ii) results of ST (p < 0.001), (iii) chloride/sodium ratio (p < 0.001), (iv) subject's age at the time of ST {[}grouped according to category (p < 0.001) and numerical order (p < 0.001)]. For the subset of 169 patients with CF and two CFTR mutations Class I, II and/or III, in comparative analysis, there was a positive association with: (i) sweat chloride/sodium ratio (p < 0.001), (ii) sweat chloride values (p = 0.047), (iii) subject's age at the time of the ST grouped by numerical order (p = 0.001). Conclusions: Considering that the quality of ST can be assessed by levels of sweat sodium and sweat chloride, an increasing number of low-quality tests could be observed in our sweat samples. The quality of the test was associated with important factors, such as gender, CF diagnosis, and subjects' age. (AU)

FAPESP's process: 11/12939-4 - Association between polymorphisms in modifier genes in children and adolescent with allergic and non-allergic: mild, moderate and severe asthma
Grantee:Fernando Augusto de Lima Marson
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/12858-5 - Identification of prevalent mutations and clinical and functional characterization of children and adults with primary ciliary dyskinesia
Grantee:Fernando Augusto de Lima Marson
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/18845-1 - Association between polymorphisms in modifier genes in children and adolescent with allergic and non-allergic mild, moderate and severe asthma
Grantee:Jose Dirceu Ribeiro
Support type: Regular Research Grants