Copaifera duckei oleoresin as a novel alternative for treatment of monogenean infections in pacu Piaractus mesopotamicus

Author(s)

da Costa, Jaqueline Custodio ; Ramos Valladao, Gustavo Moraes ; Pala, Gabriela ; Gallani, Silvia Umeda ; Kotzent, Suzana ; Miller Crotti, Antonio Eduardo ; Fracarolli, Leticia ; Mangabeira da Silva, Jonas Joaquim ; Pilarski, Fabiana

• Document type: Journal article
• Source: Aquaculture; v. 471, p. 72-79, MAR 20 2017.
• Web of Science Citations: 6

Abstract

Monogeneans are major parasites of fish and cause large economic losses in aquaculture. Treatment for this parasitic infection is done with products that are mostly toxic to fish and the environment. Essential oils (EOs) of Melaleuca alternifolia and Mentha piperita and the oleoresin (OR) of Copaifera duckei were tested for their in vitro anthelmintic activity against the monogenean parasites (Anacanthorus penilabiatus and Mymarothecium viatorum) of pacu Piaractus mesopotamicus. Naturally infected gills were bathed with the herbal solutions (100, 200, 400, 800, and 1600 mg/L) and monitored every 15 min for 4 h. Because of its greater efficacy in vitro (p < 0.05) compared to the other herbalmedicines, C. duckei ORwas selected for in vivo testing. The in vivo treatment consisted of 10 and 50 mg/L baths of C. duckei OR for 10min. Parasitological, hematological, and histological analyses were conducted post- bath and seven days after treatment. Parasite loads decreased by approximately 45% in fish treated with 50 mg/L of C. duckei OR. No hematological changes caused by treatment with C. duckei OR at 10 and 50mg/Lwere observed. Histology revealed branchial and hepatic alterations in fish from all groups, whereas spleen and kidney tissues were not affected. Histopathological alterations observed in all fish were due to parasitism or nutritional/farming conditions. Hematological and histological results showed that short baths were safe for fish. Based on the strong anthelmintic activity observed, C. duckei OR offers a promising alternative treatment against monogenean parasites. (C) 2016 Elsevier B. V. All rights reserved. (AU)

• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants