Determination of drugs in plasma samples by disposable pipette extraction with C18-BSA phase and liquid chromatography-tandem mass spectrometry

Author(s)

Lemos Pinto, Monia Ap. ; de Souza, Israel D. ; Queiroz, Maria Eugenia C.

• Document type: Journal article
• Source: Journal of Pharmaceutical and Biomedical Analysis; v. 139, p. 116-124, MAY 30 2017.
• Web of Science Citations: 10

Abstract

This work describes restricted access material (RAM) constituted of porous octadecylsilane particles with the outer surface covered with bovine serum albumin (C18-BSA) as a stationary phase to extract drugs from plasma samples by disposable pipette extraction (DPX) for further analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The C18-BSA phase simultaneously excluded macromolecules by chemical diffusion barrier (BSA network) and enrichment of the interior phase (C18) with drug traces by sorption. The hydrophilic barrier of the Cl 8-BSA allows small molecules (drugs) to permeate through the hydrophobic part (C18), while at the same time it excludes the macromolecules by chemical diffusion barrier (BSA network). Optimization of the DPX variables (sorption equilibration time, exclusion of endogenous compounds, and elution step) improved the sensitivity and selectivity of the method, which presented a linear range from the lower limit of quantification (0.5-20.0 ng mL(-1)) to the upper limit of quantification (32.5-10,500 ng mL(-1)), inter- and intra-assay precision with coefficients of variation (CV) lower than 15%, and relative standard error (RSE) of the accuracy ranging from -12% to 11%. The developed method was successfully used to determine five antipsychotics (olanzapine, quetiapine, clozapine, haloperidol, and chlorpromazine) in combination with seven antidepressants (mirtazapine, paroxetine, citalopram, sertraline, imipramine, clomipramine, and fluoxetine), two anticonvulsants (carbamazepine and lamotrigine), and two anxiolytics (diazepam and clonazepam) in plasma samples from schizophrenic patients for therapeutic drug monitoring. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 15/07619-1 - Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids and drugs in biological samples
• Grantee: Maria Eugênia Queiroz Nassur
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/03808-9 - Development of extraction phase material restricted to microextraction packed sorbent drug in plasma samples and analysis by liquid chromatography coupled to mass spectrometry sequence
• Grantee: Mônia Aparecida Lemos Pinto de Melo
• Support Opportunities: Scholarships in Brazil - Doctorate