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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oral beta-hydroxybutyrate increases ketonemia, decreases visceral adipocyte volume and improves serum lipid profile in Wistar rats

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Caminhotto, Rennan de Oliveira ; Medeiros Komino, Ayumi Cristina ; Silva, Flaviane de Fatima ; Andreotti, Sandra ; Laurato Sertie, Rogerio Antonio ; Reis, Gabriela Boltes ; Lima, Fabio Bessa
Total Authors: 7
Document type: Journal article
Source: NUTRITION & METABOLISM; v. 14, APR 24 2017.
Web of Science Citations: 6

Background: Ketosis can be induced in humans and in animals by fasting or dietary interventions, such as ketogenic diets. However, the increasing interest on the ketogenic state has motivated the development of alternative approaches to rapidly increase ketonemia using less drastic interventions. Here, it was tested whether oral intake of a beta-hydroxybutyrate (beta HB) mineral salt mixture could increase ketonemia in Wistar rats without any other dietary changes, thereby being a useful model to study ketones effects alone on metabolism. Methods: beta HB salts were orally administered to provoke elevation in the ketonemia. Effects of this intervention were tested acutely (by gavage) and chronically (4 weeks in drinking water). Acutely, a concomitant glucose overload was used to suppress endogenous ketogenesis and verify whether beta HB salts were really absorbed or not. Long-term administration allowed to weekly evaluate the impact on ketonemia, blood glucose and, after 4 weeks, on body weight, visceral fat mass, lipid blood profile, serum lipolysis products and adiponectinemia. Results: beta HB salts increased ketonemia in acute and long-term administrations, improved blood lipid profile by raising HDL-cholesterol concentration and decreasing LDL/HDL ratio, while reduced visceral adipocyte volume. Mean ketonemia correlated positively with HDLc and negatively with adipocyte volume and serum lipolysis products. Conclusions: Oral beta HB can rapidly increase ketonemia and, therefore, be used as an acute and long-term animal model of ketosis. Long-term treatment points to important beneficial effects of ketone bodies in serum lipid concentrations and visceral fat mass. These results may help to explain the metabolic adaptations following ketogenic diets, such as a better body fat control and a serum lipid profile improvement. (AU)

FAPESP's process: 14/10705-4 - Effect of beta-hydroxybutyrate in mitochondrial function of isolated white adipocytes
Grantee:Rennan de Oliveira Caminhotto
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/10218-1 - Characterization of the effects of supplementation of beta-hydroxybutyrate salts in plasma parameters, adipose tissue morphology and expression of adiponectin
Grantee:Ayumi Cristina Medeiros Komino
Support type: Scholarships in Brazil - Scientific Initiation