Low level laser therapy modulates viability, alkaline phosphatase and matrix metalloproteinase-2 activities of osteoblasts

Author(s)

de Oliveira, Flavia Amadeu ; Matos, Adriana Arruda ; Matsuda, Sandra Satiko ; Rabelo Buzalaf, Marilia Afonso ; Bagnato, Vanderley Salvador ; de Andrade Moreira Machado, Maria Aparecida ; Damante, Carla Andreotti ; de Oliveira, Rodrigo Cardoso ; Peres-Buzalaf, Camila

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 169, p. 35-40, APR 2017.
• Web of Science Citations: 6

Abstract

Low level laser therapy (LLLT) has been shown to stimulate bone cell metabolism but their impact on the matrix metalloproteinase (MMP) expression and activity is little explored. This study evaluated the influence of LLLT at two different wavelengths, red and infrared, on MC3T3-E1 preosteoblast viability, alkaline phosphatase (ALP) and MMP-2 and-9 activities. To accomplish this, MC3T3-E1 cells were irradiated with a punctual application of either red (660 nm; InGaAIP active medium) or infrared (780 nm; GaAlAs active medium) lasers both at a potency of 20 mW, energy dose of 0.08 or 0.16 J, and energy density of 1.9 J/cm(2) or 3.8 J/cm(2), respectively. The control group received no irradiation. Cellular viability, ALP and MMP-2 and-9 activities were assessed by MTT assay, enzymatic activity and zymography, respectively, at 24, 48 and 72 h. The treatment of cells with both red and infrared lasers significantly increased the cellular viability compared to the non-irradiated control group at 24 and 48 h. The ALP activity was also up modulated in infrared groups at 24 and 72 h, depending on the energy densities. In addition, the irradiation with red laser at the energy density of 1.9 J/cm(2) promoted an enhancement of MMP-2 activity at 48 and 72 h. However, no differences were observed for the MMP-9 activity. In conclusion, when used at these specific parameters, LLL modulates both preosteoblast viability and differentiation highlighted by the increased ALP and MMP-2 activities induced by irradiation. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 08/11261-1 - Evaluating the influence of laser irradiation on low intensity in the expression of RANKL and OPG in osteoblasts in vitro.
• Grantee: Adriana Arruda Matos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 10/14289-4 - Comparative study of low-intensity lasers and light emitting diode in osteogenesis.
• Grantee: Flávia Amadeu de Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 07/08367-0 - Evaluation of the influence of the irradiation with laser in low intensity in the proliferative and metabolic behavior of human osteoblasts in vitro
• Grantee: Camila Peres Buzalaf
• Support Opportunities: Regular Research Grants
• FAPESP's process: 08/11391-2 - EFFECT OF RADIATION WITH LOW INTENSITY LASER IN THE PRE-OSTEOBLAST IN VITRO: CELL VIABILITY AND MMPS -2 AND -9 EXPRESSION ANALYSIS.
• Grantee: Flávia Amadeu de Oliveira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation