Determination of Chlorophenol in Environmental Sam... - BV FAPESP
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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of Chlorophenol in Environmental Samples Using a Voltammetric Biosensor Based on Hybrid Nanocomposite

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Author(s):
Renata K. Mendes [1] ; Beatriz S. Arruda [2] ; Elizabeth F. de Souza [3] ; Alessandra B. Nogueira [4] ; Omar Teschke [5] ; Luiz O. Bonugli [6] ; Augusto Etchegaray [7]
Total Authors: 7
Affiliation:
[1] Pontíficia Universidade Católica de Campinas. Faculdade de Química - Brasil
[2] Pontíficia Universidade Católica de Campinas. Faculdade de Química - Brasil
[3] Pontíficia Universidade Católica de Campinas. Faculdade de Química - Brasil
[4] Pontíficia Universidade Católica de Campinas. Faculdade de Química - Brasil
[5] Universidade Estadual de Campinas. Instituto de Física Gleb Wataghin. Laboratório de Nanoestruturas e Interfaces - Brasil
[6] Universidade Estadual de Campinas. Instituto de Física Gleb Wataghin. Laboratório de Nanoestruturas e Interfaces - Brasil
[7] Pontíficia Universidade Católica de Campinas. Faculdade de Química - Brasil
Total Affiliations: 7
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 28, n. 7, p. 1212-1219, 2017-07-00.
Abstract

In this work, a simple electrochemical biosensor for 4-chlorophenol was developed based on laccase immobilized on a hybrid nanocomposite (ZnO nanoparticles/chitosan), and incorporated in a carbon paste electrode. There are few biosensors in the literature for this specific pollutant because it tends to form polymeric films on the electrode, causing surface passivation or even enzyme inactivation. The carbon paste allowed the surface to be easily renewed by polishing, which amends this limitation. To optimize the experimental conditions, we used cyclic voltammetry and hydroquinone as a representative of phenolic compounds due to the high toxicity of chlorophenol, thus avoiding the generation of hazardous residues. After optimization, a calibration curve was constructed for 4-chlorophenol using differential pulse voltammetry, and a linear response was obtained from 1 to 50 µM, with a lower detection limit of 0.7 µM. The obtained biosensor showed high accuracy when employed in the analysis of industrial wastewater. (AU)

FAPESP's process: 13/20570-6 - Sustainable production of biosurfactants with applications for medicine and environmental remediation
Grantee:Augusto Etchegaray Júnior
Support Opportunities: Regular Research Grants
FAPESP's process: 14/13763-5 - Determination of phenolic compounds using on voltammetric biosensor based on laccase and hybrid nanocomposite and experimental desing
Grantee:Beatriz Silva Arruda
Support Opportunities: Scholarships in Brazil - Scientific Initiation