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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy

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Author(s):
Sivieri-Araujo, Gustavo ; de Azevedo Queiroz, India Olinta ; Dal Fabbro, Renan ; Esteves, Fernanda ; Angelo Cintra, Luciano Tavares ; Tobias Duarte, Paulo Carvalho ; Bagnato, Vanderlei Salvador ; Penha Oliveira, Sandra Helena ; Gomes-Filho, Joao Eduardo
Total Authors: 9
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 18, p. 315-318, JUN 2017.
Web of Science Citations: 3
Abstract

Background: The antimicrobial photodynamic therapy (aPDT) inactivates the target cell via reactions among the photosensitizer (PS), Laser or Led and O-2. The aim of this study was to evaluate the tissue reaction and cytokine production promoted by aPDT with curcumin photosensitizer. Methods: Polyethylene tubes containing saline solution (control), 5% sodium hypochlorite (NaOCl) and aPDT with curcumin PS 500 mg/L, were implanted into dorsal connective tissue of Wistar rats. After 7, 15, 30, 60 and 90 days of implantation, the animals were euthanized and the tubes with surrounding tissues were removed. The specimens were divided in two part, one half was processed, fixed and prepared for histological analysis by staining with hematoxylin and eosin. The other half was collected for IL-1 beta and IL-6 cytokine production using ELISA assay. The results were statistically analyzed by Kruskal-Wallis test followed by Dunn test (p < 0.05) for tissue reaction and ANOVA followed by Bonferroni's correction (p < 0.05) for ELISA. Results: All groups showed severe tissue reactions at 7 days, whilst a significantly decrease by time was observed. Regarding to cytokine production, aPDT increases the IL-1 beta levels in all periods of time (p < 0.05). However, for IL-6 levels, the highest value was observed with aPDT on the 90th day (p < 0.05). Conclusions: aPDT with curcumin PS 500 mg/L demonstrated biocompatibility similar to saline solution and induced the IL-1 beta and IL-6 cytokines production. (AU)

FAPESP's process: 12/06785-7 - Influence of diferente irrigating solutions associated to photodinamic therapy: in vitro and in vivo study
Grantee:João Eduardo Gomes Filho
Support type: Regular Research Grants
FAPESP's process: 12/12696-7 - Influence of different irrigating solutions associated to photodynamic therapy: study in rats
Grantee:Fernanda Esteves
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/23287-8 - Influence of different irrigating solution associated to the photodynamic therapy: in vitro and in vivo study
Grantee:Gustavo Sivieri de Araújo
Support type: Scholarships in Brazil - Post-Doctorate