Copaifera multijuga oleoresin and its constituent diterpene (-)-copalic acid: Genotoxicity and chemoprevention study

Author(s)

Alves, Jacqueline M. ; Senedese, Juliana M. ; Leandro, Luis F. ; Castro, Pamela T. ; Pereira, Daiane E. ; Carneiro, Luiza J. ; Ambrosio, Sergio R. ; Bastos, Jairo K. ; Tavares, Denise C.

• Document type: Journal article
• Source: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 819, p. 26-30, JUL 2017.
• Web of Science Citations: 5

Abstract

Copaiba oleoresins are used in alternative medicine as anti-inflammatory, antitumoral, and antimicrobial treatments. (-)-Copalic acid (CA) is the major diterpene found in exudates from Copaifera species. We have examined the genotoxicity and the chemopreventive potential of Copaifera multijuga oleoresin (CM) and CA. Genotoxicity assessment was examined with the peripheral blood micronucleus test and the comet assay (male Swiss mouse hepatocytes). In the chemoprevention study, we evaluated the effects of CM and CA on the formation of 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) in male Wistar rat colon. Neither agent caused a significant increase in micronucleus frequency relative to controls, but the highest CM dose tested (400 mg/kg b.w.) caused DNA damage in the comet assay. Both agents significantly reduced the frequency of DMH-induced ACF. Both CM and CA suppressed ACF formation and may have a protective effect against colon carcinogenesis. (AU)

• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 09/17237-8 - In vivo and in vitro evaluation of cytotoxic, mutagenic, genotoxic potential and chemopreventive effect of Copaifera langsdorffii oleoresin and copalic acid diterpenoid
• Grantee: Jacqueline Morais Alves
• Support Opportunities: Scholarships in Brazil - Doctorate