Revisiting Coleoptera a plus T-rich region: structural conservation, phylogenetic and phylogeographic approaches in mitochondrial control region of bioluminescent Elateridae species (Coleoptera)

Author(s)

Amaral, Danilo T. ; Mitani, Yasuo ; Oliveira, Gabriela ; Ohmiya, Yoshihiro ; Viviani, Vadim R.

• Document type: Journal article
• Source: MITOCHONDRIAL DNA PART A; v. 28, n. 4-5, p. 671-680, SEP 2017.
• Web of Science Citations: 4

Abstract

The control region (CR) or A + FT-rich region in Coleoptera mt genome is poorly characterized, including the Elateroidea bioluminescent species. Here, we provided the first attempt to characterize and compare the structure and organization of the CR of different species within Elateridae. We also revisited some sequenced Coleoptera CR and observed consensus T-stretches, non-conserved sequences near the stem-loop and unusual inner tRNAs-like sequences. All these features are probably involved in the replication start of the mt genome. The phylogenetic relationships in Elateridae bioluminescent groups using partial sequence of CR showed the monophyly of Pyrearinus pumilus group and Pyrearinus as a polyphyletic genus, corroborating our previous results. The wider genetic variation obtained by CR analysis could separate two different lineages that occur within P. termitilluminans populations. In Elateridae, the CR exhibited high polymorphism within and between populations, which was also observed in other Coleoptera species, suggesting that the CR could be described as a suitable molecular marker to be applied in phylogenetic and phylogeographic studies. (AU)

• FAPESP's process: 14/20176-9 - Transcriptome of larva and adult lanterns and larvae fatty body of Elateroidea (Coleoptera)
• Grantee: Danilo Trabuco do Amaral
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/00574-7 - Organization and comparative analysis of the mitochondrial genome for phylogenetic studies in Elateroidea (Coleoptera: Polyphaga)
• Grantee: Danilo Trabuco do Amaral
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• FAPESP's process: 13/09594-0 - Analysis of coleopteran lanterns, fat body and Malpighian tubules transcriptomes, and its relationship with the origins of bioluminescence in Coleoptera and Diptera
• Grantee: Vadim Viviani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/19427-9 - Bioluminescent beetles from the Atlantic Rain Forest, Cerrado and transition to Amazon Forest: biodiversity, molecular evolution and phylogeny, and use as bioindicators
• Grantee: Vadim Viviani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/02868-0 - Molecular evolution of the luciferases of the lanterns from the adult and larval stages of luminescent elaterids and molecular phylogeny of Elateroidea
• Grantee: Danilo Trabuco do Amaral
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)