Fluorescence lifetime evaluation of whole soils from the Amazon rainforest

Author(s)

Nicolodelli, Gustavo ; Tadini, Amanda Maria ; Nogueira, Marcelo Saito ; Pratavieira, Sebastiao ; Mounier, Stephane ; Clabel Huaman, Jose Luis ; dos Santos, Cleber Hilario ; Montes, Celia Regina ; Bastos Pereira Milori, Debora Marcondes

• Document type: Journal article
• Source: APPLIED OPTICS; v. 56, n. 24, p. 6936-6941, AUG 20 2017.
• Web of Science Citations: 3

Abstract

Time-resolved fluorescence spectroscopy (TRFS) is a new tool that can be used to investigate processes of interaction between metal ions and organic matter (OM) in soils, providing a specific analysis of the structure and dynamics of macromolecules. To the best of our knowledge, there are no studies in the literature reporting the use of this technique applied to whole/non-fractionated soil samples, making it a potential method for use in future studies. This work describes the use of TRFS to evaluate the fluorescence lifetimes of OM of whole soils from the Amazon region. Analysis was made of pellets of soils from an oxisol-spodosol system, collected in Sao Gabriel da Cachoeira (Amazonas, Brazil). The fluorescence lifetimes in the oxisol -spodosol system were attributed to two different fluorophores. One was related to complexation of an OM fraction with metals, resulting in a shorter fluorophore lifetime. A short fluorescence lifetime (2-12 ns) could be associated with simpler structures of the OM, while a long lifetime (19-66 ns) was associated with more complexOM structures. This new TRFS technique for analysis of the fluorescence lifetime in whole soil samples complies with the principles of green chemistry. (C) 2017 Optical Society of America (AU)

• FAPESP's process: 12/24349-0 - Development and evaluation of a double-pulse LIBS system: Application for soil characterization
• Grantee: Gustavo Nicolodelli
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 11/03250-2 - Quantification and dynamics of carbon associated with deep spodic horizons of Amazon Forest soils, towards possible climatic changed
• Grantee: Célia Regina Montes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/13013-3 - Characterization Humic Substances extracted from Amazonian Soils
• Grantee: Amanda Maria Tadini
• Support Opportunities: Scholarships in Brazil - Doctorate