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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Titanium-released from dental implant enhances pre-osteoblast adhesion by ROS modulating crucial intracellular pathways

Full text
Author(s):
Rossi, M. C. [1] ; Bezerra, F. J. B. [1] ; Silva, R. A. [1] ; Crulhas, B. P. [1] ; Fernandes, C. J. C. [1] ; Nascimento, A. S. [1] ; Pedrosa, V. A. [1] ; Padilha, P. [1] ; Zambuzzi, W. F. [1]
Total Authors: 9
Affiliation:
[1] State Univ Sao Paulo UNESP, Dept Chem & Biochem, Biosci Inst, Campus Botucatu, POB 510, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Biomedical Materials Research Part A; v. 105, n. 11, p. 2968-2976, NOV 2017.
Web of Science Citations: 11
Abstract

It is important to understand the cellular and molecular events that occur at the cell-material interface of implants used for bone repair. The mechanisms involved in the initial stages of osteoblast interactions with the surface of the implant material must be decisive for cell fating surrounding them. In order to address this issue, we decided to investigate if conditioned medium for dental implants was able to modulate murine pre-osteoblast metabolism. First, we determined the concentration of titanium (Ti)-containing conditioned medium and found that it was 2-fold increased (p<0.0001). We have reported that this conditioned medium significantly up-modulated pre-osteoblast adhesion up to 24 h (p<0.0001). In parallel, our results showed that both phosphorylations of FAK (focal adhesion kinase) at Y397 (p<0.0011) and Cofilin at Ser03 (p<0.0053) were also up-modulated, as well as for Rac1 expression (p<0.0175); both of them are involved with cell adaptation by rearranging cytoskeleton actin filaments. Thereafter, Ti-containing medium stimulated ROS (reactive oxygen species) production by pre-osteoblast cells, and it is very possible that ROS compromised PTP-1B (protein tyrosine phosphatase 1B) activation since PTP1B was down-phosphorylated (p<0.0148). The low PTP activity guarantees the phosphorylation of FAK at Y-residue, causing better pre-osteoblast adhesion in response to Ti-containing medium. Altogether, these data indicate that ROS indirectly modulate FAK phosphorylation in response to Ti-released from dental implants. Taken the results in account, these data showed for the first time that the implanted dental device is able to dynamically affect surrounding tissues, mainly by promoting a better performance of the pre-osteoblast cells. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2968-2976, 2017. (AU)

FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Research Grants - Young Investigators Grants