Determination of Tetracycline in Bovine and Breast Milk Using a Graphite-Polyurethane Composite Electrode

Author(s)

Fioramonti Calixto, Carolina Maria ^[1] ; Gomes Cavalheiro, Eder Tadeu ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Analytical Letters; v. 50, n. 14, p. 2323-2334, 2017.
• Web of Science Citations: 2

Abstract

A bare graphite-polyurethane composite electrode (60% graphite, m/m) was used for the determination of tetracycline in bovine and breast milk. Limits of detection as low as 2.6 mu molL(-1) were obtained in pH 2.3 phosphate buffer using optimized differential pulse voltammetric parameters at the graphite-polyurethane composite electrode. The milk treatment consisted of acidification with 80% trichloroacetic acid, centrifugation to remove solid phase from milk, the isolation of the antibiotic from the whey using solid-phase extraction, and direct analysis in pH 2.3 phosphate buffer. Recoveries between 83 and 99% and 97% were obtained for tetracycline fortified in bovine and breast milk, suggesting that this approach is a promising screening procedure. (AU)

• FAPESP's process: 08/02590-1 - Using composite electrodes in the voltammetric determination of pharmaceuticals in biological and/or environmental samples.
• Grantee: Carolina Maria Fioramonti Calixto
• Support Opportunities: Scholarships in Brazil - Doctorate