In vitro toxicological assessment of Arrabidaea brachypoda (DC.) Bureau: Mutagenicity and estrogenicity studies

Author(s)

Resende, Flavia Aparecida ^[1] ; Nogueira, Catarine Haide ^[2] ; Espanha, Livia Greghi ^[2] ; Boldrin, Paula Karina ^[2] ; Oliveira-Hohne, Ana Paula ^[2] ; de Camargo, Mariana Santoro ^{[1, 3]} ; da Rocha, Claudia Quintino ^[4] ; Vilegas, Wagner ^[5] ; Varanda, Eliana Aparecida ^[2]

Affiliation

^[1] UNIARA Univ Araraquara, Dept Biol Sci & Hlth, BR-14801340 Sao Paulo - Brazil

^[2] UNESP Sao Paulo State Univ, Fac Pharmaceut Sci Araraquara, Dept Biol Sci, BR-14801902 Sao Paulo - Brazil

^[3] UFSCAR Fed Univ Sao Carlos, CCET, BR-13565905 Sao Carlos, SP - Brazil

^[4] UFMA Fed Univ Maranhao, CCET, Dept Chem, BR-65065545 Sao Luis - Brazil

^[5] UNESP Sao Paulo State Univ, Expt Campus Paulista Coast, BR-11330900 Sao Paulo - Brazil

• Document type: Journal article
• Source: REGULATORY TOXICOLOGY AND PHARMACOLOGY; v. 90, p. 29-35, NOV 2017.
• Web of Science Citations: 1

Abstract

Arrabidaea brachypoda (DC.) Bureau is a shrub native Cerrado, known as ``cipo-una{''}, ``tintureiro{''} or ``cervejinha do campo{''} and popularly used in Southeastern and Northeastern Brazil to treatment of kidney stones and painful joints (arthritis). Nevertheless, scientific information regarding this species is scarce, and there are no reports related to its possible estrogenic and mutagenic effects. Thus, the principal objective of this study was to assess the mutagenic and estrogenic activities of the hydro alcoholic extracts of the leaves, stalks, roots, their respective fractions and isolated compounds of A. brachypoda. The mutagenic activity was evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, in the absence (-S9) and presence (+S9) of metabolic activation system. In the RYA was used Saccharomyces cerevisiae engineered strain BY4741 (MATaura3 Delta 0 leu2 Delta 0 his3 Delta 1 met15 Delta 0) which reproduce the natural pathway of genetic control by estrogens in vertebrate cells; it has the advantage of its simplicity and a high throughput. All extracts and aqueous fraction of leaves A. brachypoda were mutagenic. The crude extract is more active than the fraction, suggesting a synergic effect. Only hydroalcoholic extracts of leaves and roots of A. brachypoda showed significant estrogenic activity, with ER alpha-dependent transcriptional activation activity. The obtained results in this study showed the presence of compounds capable of interacting with the estrogen receptor and to induce damage in the genetic material. Thus, we demonstrated the risk which the population is subjected due to indiscriminate use of extracts without detailed study. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
• Grantee: Wagner Vilegas
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants