Melatonin and IL-25 modulate apoptosis and angiogenesis mediators in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumour cells

Author(s)

Gelaleti, G. B. ^{[1, 2]} ; Borin, T. F. ^[3] ; Maschio-Signorini, L. B. ^[1] ; Moschetta, M. G. ^[1] ; Hellmen, E. ^[4] ; Viloria-Petit, A. M. ^[5] ; Zuccari, D. A. P. C. ^{[1, 2]}

Affiliation

^[1] Fac Med Sao Jose do Rio Preto FAMERP, LIMC, Sao Jose Do Rio Preto - Brazil

^[2] Univ Estadual Paulista Julio de Mesquita Filho UN, Programa Posgrad Genet, IBILCE, Sao Jose Do Rio Preto - Brazil

^[3] Augusta Univ, Georgia Canc Ctr, Tumor Imaging Angiogenesis Lab, Augusta, GA - USA

^[4] Swedish Univ Agr Sci, Fac Vet Med, Dept Anat Physiol & Biochem, Uppsala - Sweden

^[5] Univ Guelph, Dept Biomed Sci, Ontario Vet Coll, Room 3647, 50 Stone Rd East, Guelph, ON N1G 2W1 - Canada

• Document type: Journal article
• Source: VETERINARY AND COMPARATIVE ONCOLOGY; v. 15, n. 4, p. 1572-1584, DEC 2017.
• Web of Science Citations: 2

Abstract

Background: Melatonin has oncostatic actions and IL-25 is active in inflammatory processes that induce apoptosis in tumor cells Aim: The aim of this study was to evaluate melatonin and IL-25 in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumor cells cultured as monolayers and tridimensional structures. Materials and Methods: The cells were treated with melatonin, IL-25 and IL-17B silencing gene and performed cell viability, gene and protein expression of caspase-3 and VEGFA (Vascular endothelial growth factor A) and an apoptosis membrane protein array. Results: Treatment with 1 mM of melatonin reduced cell viability of both tumor cell lines, all treatments alone and combined significantly increased caspase-3 cleaved and proteins involved in the apoptotic pathway and reduced pro-angiogenic VEGFA, confirming the effectiveness of these potential promising treatments. Conclusion: This is the first study evaluating the potential use of these strategies in CF-41 and CMT-U229 cell lines and together encourages subsequent in vitro and in vivo studies for further exploration of clinical applications. (AU)

• FAPESP's process: 12/02128-1 - Modulation of Interleukin 17B and 25 Activity Associated with Melatonin as Inductor of Apoptosis in Cell Culture of Mammary Neoplasms
• Grantee: Gabriela Bottaro Gelaleti
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/06098-0 - Modulation of interleukin 17b and 25 activity associated with melatonin as inductor of apoptosis in cell culture of mammary neoplasms
• Grantee: Debora Aparecida Pires de Campos Zuccari
• Support Opportunities: Regular Research Grants