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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A simple reversed phase high-performance liquid chromatography (HPLC) method for determination of in situ gelling curcumin-loaded liquid crystals in in vitro performance tests

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Fonseca-Santos, Bruno [1] ; Daflon Gremiao, Maria Palmira [1] ; Chorilli, Marius [1]
Total Authors: 3
[1] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Drugs & Med, UNESP, Campus Araraquara, Rodovia Araraquara Jan, Km 1, BR-14801902 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ARABIAN JOURNAL OF CHEMISTRY; v. 10, n. 7, p. 1029-1037, NOV 2017.
Web of Science Citations: 5

A simple, rapid, and sensitive analytical procedure has been developed and validated for the in vitro measurement of curcumin in samples from mucosae retention studies. Curcumin was analyzed by HPLC using a C-18 column with UV detection at 425 nm. The mobile phase was acetonitrile and water (50:50 v/v) acidified with 2% acetic acid at a flow rate of 1.2 mL min(-1). The curve range was linear for the receptor solution concentration range 0.5- 75 mu g mL(-1). The specificity showed no interference with the biological matrix and excipients of the acceptor media. Intra and inter-day accuracy, and precision values were lower than 5% and were not statically different (P < 0.05). Recoveries ranged from 99.7% to 108%. The limits of detection and quantitation were 11.61 and 500 ng mL(-1), respectively. The method is adequate to assay curcumin from esophageal porcine samples, enabling the determination of penetration profiles for in situ gelling curcumin-loaded liquid crystals by in vitro studies, and fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of curcumin in porcine esophageal mucosae. (C) 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. (AU)

FAPESP's process: 13/03746-3 - Mucoadhesive precursor systems of liquid crystals for buccal administration of curcumin in the treatment of oral cancer
Grantee:Bruno da Fonseca dos Santos
Support type: Scholarships in Brazil - Master