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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Susceptibility of mice to bovine herpesvirus type 5 infection in the central nervous system

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Author(s):
Mesquita, L. P. [1] ; Costa, R. C. [1] ; Fusuma, M. M. [2] ; Bruhn, F. R. P. [3] ; Mori, E. [4] ; Pituco, E. M. [2] ; Mori, C. M. C. [1] ; Weiblen, R. [5] ; Maiorka, P. C. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil
[2] Biol Inst, Lab Bovine Viral Dis, Sao Paulo - Brazil
[3] Univ Fed Pelotas, Coll Vet Med, Pelotas - Brazil
[4] Pasteur Inst, Sao Paulo - Brazil
[5] Univ Fed Santa Maria, Dept Vet Prevent Med, Santa Maria, RS - Brazil
Total Affiliations: 5
Document type: Journal article
Source: VETERINARY RESEARCH COMMUNICATIONS; v. 41, n. 4, p. 279-288, DEC 2017.
Web of Science Citations: 0
Abstract

Bovine herpesvirus type 5 (BoHV-5) is an important pathogen that causes meningoencephalitis in cattle. Few studies have used the mouse as a model for BoHV-5 infection. Despite the fact that BoHV-5 can infect mice with immune deficiencies, little is known about viral replication, immune response, and the course of infection in the central nervous system (CNS) of wild-type mice. Therefore, the aim of this study was to evaluate the response in the CNS of BALB/c mice acutely infected with BoHV-5 at different days post-inoculation (dpi). BoHV-5, when inoculated intracranially, was able to infect and replicate within the CNS of BALB/c mice. Until 15 dpi, the mice were able to survive without showing prominent neurological signs. The infection was accompanied by a Th1 immune response, with a significant expression of the cytokines IFN-gamma and TNF-alpha and chemokine CCL-2. The expression of these cytokines and chemokines was most significant in the early course of infection (3 and 4 dpi), and it was followed by meningoencephalitis with perivascular cuffing and periventriculitis, composed mainly of macrophages and lymphocytes. After the expression of cytokines and chemokine, the mice were able to curb BoHV-5 acute infection in the brain, since there was a decrease in the number of BoHV-5 DNA copies after 3 dpi and viable viral particles were not detected after 6 dpi. Importantly, BoHV-5 was able to infect the trigeminal ganglia during acute infection, since a large number of BoHV-5 DNA copies were detected on 1 and 2 dpi. (AU)

FAPESP's process: 12/24769-9 - Identification of markers of imune and inflammatory response in experimentally induced viral encephalitis in a murine model
Grantee:Paulo César Maiorka
Support Opportunities: Regular Research Grants
FAPESP's process: 13/00629-6 - Evaluation of Immune and Inflammatory Responses in Experimentally Induced Encephalitis in Mice by Bovine Herpesvirus 5 (BHV-5) and Vesicular Estomatitis Virus (VSV) Infection.
Grantee:Leonardo Pereira Mesquita
Support Opportunities: Scholarships in Brazil - Doctorate