Validity and reliability of incremental test to determine the anaerobic threshold in swimming rats

Author(s)

Beck, Wladimir R. ^[1] ; Campesan, Yuri S. ^[1] ; Gobatto, Claudio A. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Sport Sci, Sch Appl Sci, Lab Appl Sport Physiol, BR-13484350 Sao Paulo - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF APPLIED EXERCISE PHYSIOLOGY; v. 4, n. 1, p. 25-33, APR 2015.
• Web of Science Citations: 2

Abstract

The organic and metabolic consequences of physical exercise are critically modulated depending of the effort intensity and volume. Nevertheless, most of the protocols employed for objectively determine the individual exercise intensities for rats are impractical or unadvisable for various experimental designs. The aim of this study was to individually determine the anaerobic threshold intensity (iAnT) and the maximal lactate steady state (MLSS, gold standard procedure) intensities using a single incremental swimming test for rats, verifying it validity and reliability. Eleven male Wistar rats were submitted twice (48 hours of interval) to an incremental test with overloads from 3% of body mass (% bm), increments of 0.5% bm and stages of 5 min. From blood lactate concentration and % bm data were constructed two linear regressions to determine iAnT. Then, all of the animals performed the MLSS procedure based on iAnT. Comparing iAnT test and re-test were found significant intraclass correlation (r = 0.67; p = 0.01), no significant difference (p = 0.91), coefficient of variation of 4.04% and effect size of 0.02, beyond good agreement, precision and accuracy attested by Bland-Altman plots (bias of 0.010). Furthermore, iAnT was not different from MLSS intensity. A single incremental swimming test comprises a high applicable, valid and reliable tool for objectively and individually determines exercise intensity of swimming rats. (AU)

• FAPESP's process: 09/08535-5 - Standardization and validity of aerobic and anaerobic tests in field and laboratory, using free, tethered and half-tethered running, in endurance and sprint runners
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/13377-7 - Effects of light and time of day of swimming exercise until exhaustion at lactate minimum intensity on biochemistry and hematologic responses of rats
• Grantee: Wladimir Rafael Beck
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/20501-1 - Molecular responses of continuous and intermitent swimming and of exogenous melatonin in rats: cell signalizers of the intermediary metabolism and from the inflammatory and oxidative stress pathways
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/13226-1 - Ergogenic response of melatonin during the nadir and acrophase of spontaneous activity: consequences on IKK/Nf-kB via
• Grantee: Wladimir Rafael Beck
• Support Opportunities: Scholarships in Brazil - Doctorate