| Full text | |
| Author(s): Show less - |
Diniz, Ivana M. A.
[1]
;
Carreira, Ana C. O.
[2, 3]
;
Sipert, Carla R.
[4]
;
Uehara, Cindi M.
[4]
;
Moreira, Maria S. N.
[5]
;
Freire, Laila
[4]
;
Pelissari, Cibele
[6]
;
Kossugue, Patricia M.
[2, 3]
;
de Araujo, Daniele R.
[7]
;
Sogayar, Mari C.
[2, 3]
;
Marques, Marcia M.
[4]
Total Authors: 11
|
| Affiliation: | [1] Univ Fed Minas Gerais, Dept Restorat Dent, Sch Dent, Ave Antonio Carlos 6627, BR-31279910 Belo Horizonte, MG - Brazil
[2] Univ Sao Paulo, Cell & Mol Therapy Ctr NUCEL NETCEM, Sch Med, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Biochem, Chem Inst, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo - Brazil
[5] Univ Ibirapuera, Dept Biodent, Sch Dent, Sao Paulo - Brazil
[6] Univ Sao Paulo, Dept Stomatol, Sch Dent, Sao Paulo - Brazil
[7] ABC Fed Univ, Nat & Human Sci Ctr, Santo Andre - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Journal of Cellular Physiology; v. 233, n. 6, p. 4907-4918, JUN 2018. |
| Web of Science Citations: | 9 |
| Abstract | |
Photobiomodulation (PBM) therapy displays relevant properties for tissue healing and regeneration, which may be of interest for the tissue engineering field. Here, we show that PBM is able to improve cell survival and to interact with recombinant human Bone Morphogenetic Protein 4 (rhBMP4) to direct and accelerate odonto/osteogenic differentiation of dental derived mesenchymal stem cells (MSCs). MSCs were encapsulated in an injectable and thermo-responsive cell carrier (Pluronic((R)) F-127) loaded with rhBMP4 and then photoactivated. PBM improved MSCs self-renewal and survival upon encapsulation in the Pluronic((R)) F-127. In the presence of rhBMP4, cell odonto/osteogenic differentiation was premature and markedly improved in the photoactivated MSCs. An in vivo calvarial critical sized defect model demonstrated significant increase in bone formation after PBM treatment. Finally, a balance in the reactive oxygen species levels may be related to the favorable results of PBM and rhBMP4 association. PBM may act in synergism with rhBMP4 and is a promise candidate to direct and accelerate hard tissue bioengineering. (AU) | |
| FAPESP's process: | 12/16552-0 - Effects of low level laser therapy associated to a BMP4 incorporated scaffold on dental pulp stem-cells differentiation and migration. An in vitro study. |
| Grantee: | Ivana Marcia Alves Diniz |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/17268-6 - Effect of laser phototherapy on the transplantation of a BMP4-loaded hydrogel plus stem cells for dentin-pulp complex regeneration in vivo |
| Grantee: | Ivana Marcia Alves Diniz |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |