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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Melatonin improves survival and respiratory activity of yeast cells challenged by alpha-synuclein and menadione

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Author(s):
Zampol, Mariana A. [1] ; Barros, Mario H. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: YEAST; v. 35, n. 3, p. 281-290, MAR 2018.
Web of Science Citations: 4
Abstract

One of the hallmarks of Parkinson disease is -synuclein aggregate deposition that leads to endoplasmic reticulum stress, Golgi fragmentation and impaired energy metabolism with consequent redox imbalance. In the last decade, many studies have used Saccharomyces cerevisiae as a model in order to explore the intracellular consequences of -synuclein overexpression. In this study we propose to evaluate the respiratory outcome of yeast cells expressing -synuclein. Cell viability or growth on selective media for respiratory activity was mainly affected in the -synuclein-expressing cells if they were also treated with menadione, which stimulates reactive oxygen species production. We also tested whether melatonin, a natural antioxidant, would counteract the deleterious effects of -synuclein and menadione. In fact, melatonin addition improved the respiratory growth of -synuclein/menadione-challenged cells, presented a general improvement in the enzymatic activity of the respiratory complexes and finally elevated the rate of mitophagy, an important cellular process necessary for the clearance of damaged mitochondria. Altogether, our data confirms that -synuclein impairs respiration in yeast, which can be rescued by melatonin addition. (AU)

FAPESP's process: 11/07366-5 - Study of mitochondrial proteins of unknown function and their respective effects on cell viability
Grantee:Mario Henrique de Barros
Support Opportunities: Regular Research Grants
FAPESP's process: 11/03583-1 - Natural antioxidants action in Saccharomyces cerevisiae parkinsonian model
Grantee:Mariana Alba Zampol
Support Opportunities: Scholarships in Brazil - Master