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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Vibrational spectroscopy of muscular tissue intoxicated by snake venom and exposed to photobiomodulation therapy

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Author(s):
Vieira, Willians Fernando [1, 2] ; Kenzo-Kagawa, Bruno [1] ; Mesquita Britto, Maria Helena [1] ; Ceragioli, Helder Jose [2] ; Sakane, Kumiko Koibuchi [3] ; Baranauskas, Vitor [2] ; da Cruz-Hofling, Maria Alice [1]
Total Authors: 7
Affiliation:
[1] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13083970 Campinas, SP - Brazil
[2] State Univ Campinas UNICAMP, Fac Elect Engn & Computat, Dept Semicond Instruments & Photon, BR-13083852 Campinas, SP - Brazil
[3] Univ Vale Paraiba Univap, Inst Res & Dev, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Lasers in Medical Science; v. 33, n. 3, p. 503-512, APR 2018.
Web of Science Citations: 1
Abstract

The pathogenesis of myonecrosis caused by myotoxins from bothropic venom is associated with local extracellular matrix (ECM) disintegration, hemorrhage, and inflammation. Search for alternative methods associated with serum therapy is mandatory to neutralize the fast development of local damage following snakebites. The experimental use of photobiomodulation therapy (PBMT) in murine models has shown promising results relative to structural and functional recovery from bothropic snakebite-induced myonecrosis. This study pioneered in using Raman and Fourier transform infrared (FTIR) spectroscopies to characterize biochemical alterations in the gastrocnemius that had been injected with Bothrops jararacussu venom and exposed to local PBMT. Results show that vibrational spectra from lyophilized and diluted venom (1307 cm (-1)) was also found in the envenomed gastrocnemius indicating venom presence in the unirradiated muscle 48 h post-injection; but any longer visible after PBMT at this time exposure or 72 h post-injection regardless irradiated or not. Raman and FTIR analyses indicated that the bands with higher area and intensity were 1657 and 1547 cm(-1) and 1667 and 1452 cm(-1), respectively; all are assignments for proteins, especially collagen, and are higher in the PBMT-exposed gastrocnemius. The infrared spectra suggest that laser treatment was able to change protein in tissue and that such change indicates collagen as the main target. We hypothesize that the findings reflect remodeling of ECM with key participation of collagen and faster tissue recovery for an anabolic condition. (AU)