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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lignolytic-consortium omics analyses reveal novel genomes and pathways involved in lignin modification and valorization

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Moraes, Eduardo C. [1] ; Alvarez, Thabata M. [2] ; Persinoti, Gabriela F. [1] ; Tomazetto, Geizecler [1] ; Brenelli, Livia B. [1] ; Paixao, Douglas A. A. [1] ; Ematsu, Gabriela C. [1] ; Aricetti, Juliana A. [1] ; Caldana, Camila [1] ; Dixon, Neil [3] ; Bugg, Timothy D. H. [4] ; Squina, Fabio M. [5]
Total Authors: 12
[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol, Campinas - Brazil
[2] UP, Master Program Ind Biotechnol, Curitiba, Parana - Brazil
[3] Univ Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester, Lancs - England
[4] Univ Warwick, Dept Chem, Coventry, W Midlands - England
[5] Univ Sorocaba, Programa Proc Tecnol & Ambientais, Sorocaba - Brazil
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 10

Background: Lignin is a heterogeneous polymer representing a renewable source of aromatic and phenolic bioderived products for the chemical industry. However, the inherent structural complexity and recalcitrance of lignin makes its conversion into valuable chemicals a challenge. Natural microbial communities produce biocatalysts derived from a large number of microorganisms, including those considered unculturable, which operate synergistically to perform a variety of bioconversion processes. Thus, metagenomic approaches are a powerful tool to reveal novel optimized metabolic pathways for lignin conversion and valorization. Results: The lignin-degrading consortium (LigMet) was obtained from a sugarcane plantation soil sample. The LigMet taxonomical analyses (based on 16S rRNA) indicated prevalence of Proteobacteria, Actinobacteria and Firmicutes members, including the Alcaligenaceae and Micrococcaceae families, which were enriched in the LigMet compared to sugarcane soil. Analysis of global DNA sequencing revealed around 240,000 gene models, and 65 draft bacterial genomes were predicted. Along with depicting several peroxidases, dye-decolorizing peroxidases, laccases, carbohydrate esterases, and lignocellulosic auxiliary (redox) activities, the major pathways related to aromatic degradation were identified, including benzoate (or methylbenzoate) degradation to catechol (or methylcatechol), catechol orthocleavage, catechol meta-cleavage, and phthalate degradation. A novel Paenarthrobacter strain harboring eight gene clusters related to aromatic degradation was isolated from LigMet and was able to grow on lignin as major carbon source. Furthermore, a recombinant pathway for vanillin production was designed based on novel gene sequences coding for a feruloyl-CoA synthetase and an enoyl-CoA hydratase/aldolase retrieved from the metagenomic data set. Conclusion: The enrichment protocol described in the present study was successful for a microbial consortium establishment towards the lignin and aromatic metabolism, providing pathways and enzyme sets for synthetic biology engineering approaches. This work represents a pioneering study on lignin conversion and valorization strategies based on metagenomics, revealing several novel lignin conversion enzymes, aromatic-degrading bacterial genomes, and a novel bacterial strain of potential biotechnological interest. The validation of a biosynthetic route for vanillin synthesis confirmed the applicability of the targeted metagenome discovery approach for lignin valorization strategies. (AU)

FAPESP's process: 13/03061-0 - Characterization and antioxidant capacity of fractions of lignin extracted from sugarcane bagasse
Grantee:Lívia Beatriz Brenelli de Paiva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/26152-4 - Enzyme prospecting and metabolic pathway optimization for vanillin production from sugar cane bagasse lignin
Grantee:Eduardo Cruz Moraes
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
Grantee:Fábio Márcio Squina
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 15/23279-6 - Metagenomic and metasecretomic analyses of an enrichment microbial consortium of anaerobic fungi to the degradation of sugarcane bagasse: prospection of cellulosomas and lignocellolytic enzymes
Grantee:Geizecler Tomazetto
Support type: Scholarships in Brazil - Post-Doctorate