Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of short-term dietary restriction and glutamine supplementation in vitro on the modulation of inflammatory properties

Full text
Author(s):
de Oliveira, Dalila C. [1] ; Santos, Ed Wilson [1] ; Nogueira-Pedro, Amanda [1] ; Xavier, Jose Guilherme [2] ; Borelli, Primavera [1] ; Fock, Ricardo Ambrosio [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
[2] Univ Paulista, Inst Hlth Sci, Sch Vet Med, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: NUTRITION; v. 48, p. 96-104, APR 2018.
Web of Science Citations: 1
Abstract

Objective: Dietary restriction (DR) is a nutritional intervention that exerts profound effects on biochemical and immunologic parameters, modulating some inflammatory properties. Glutamine (GLN) is a conditionally essential amino acid that can modulate inflammatory properties. However, there is a lack of data evaluating the effects of DR and GLN supplementation, especially in relation to inflammatory cytokine production and the expression of transcription factors such as nuclear factor (NF)-kappa B. Methods: We subjected 3-mo-old male Ballb/c mice to DR by reducing their food intake by 30%. DR animals lost weight and showed reduced levels of serum triacylglycerols, glucose, cholesterol, and calcium as well as a reduction in bone density. Additionally, blood, peritoneal, and spleen cellularity were reduced, lowering the number of peritoneal F4/80- and CD86-positive cells and the total number of splenic CD4- and CD8-positive cells. Results: The production of interleukin (IL)-10 and the expression of NF-kappa B in splenic cells were not affected by DR or by GLN supplementation. However, peritoneal macrophages from DR animals showed reduced IL-12 and tumor necrosis factor-alpha production and increased IL-10 production with reduced phosphorylation of NF-kappa B expression. Additionally, GLN was able to modulate cytokine production by peritoneal cells from the control group, although no effects were observed in cells from the DR group. Conclusion: DR induces biochemical and immunologic changes, in particular by reducing IL-12 and tumor necrosis factor-alpha production by macrophages and clearly upregulating IL-10 production, whereas GLN supplementation did not modify these parameters in cells from DR animals. (C) 2017 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 16/16463-8 - Evaluation of modulatory effects and mechanisms of the synthetic fragment of leptin LEP5 in the hematopoiesis regulaton in mice submitted to protein malnutrition
Grantee:Ricardo Ambrósio Fock
Support Opportunities: Regular Research Grants