Evaluation of modulatory effects and mechanisms of the synthetic fragment of leptin LEP5 in the hematopoiesis regulaton in mice submitted to protein malnutrition

• Grant number: 16/16463-8
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2016 - November 30, 2018
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Ricardo Ambrósio Fock
• Grantee: Ricardo Ambrósio Fock
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Amanda Nogueira Pedro ; Primavera Borelli Garcia
• Associated grant(s): 17/50022-1 - The role of age-associated endothelial dysfunction in hematopoiesis, AP.R SPRINT

Abstract

Malnutrition is considered a disease responsible for 55% of children deaths worldwide, mainly under five years old. The FAO (Food and Agriculture Organization) says that in 2012 there were about 86 million malnourished people worldwide and protein malnutrition (PM) is the most common. It is known that the PM causes hematological disorders such as anemia and histological changes in the bone marrow extracellular matrix, affecting bone marrow microenvironment and impairing the polimorphonuclear cells production and functions. The bone marrow microenvironment plays an essential role in production of several hematopoietics modulators; such as growth factors, cytokines which are able to modulate proliferation, differentiation, adhesion and migration of hematopoietic cells. The leptin is a protein, known as satiety hormone, however previous studies reported some hematopoietic biological effects of this protein. Thus, studies related to leptin peptide fragments, bioactive, have been of great importance, since they are easier to produce and inexpensive. Thus, in previous studies with LEP5 fragment Ac- [Ser117] -hLEP116-140-NH2 was demonstrated its bioactivity, because it increased the amount of hematopoietic stem cells in mice. Knowing that the hematopoietic system of PM animals are compromised and fragment LEP5 displays some biological activities on the hematopoietic system, we intend to study the toxic effects of this fragments in vivo and how it can modulate the hematopoiesis system, evaluating the mechanism involved in this regulation. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SARTORI, TALITA; DOS SANTOS, GUILHERME GALVAO; NOGUEIRA-PEDRO, AMANDA; MAKIYAMA, EDSON; ROGERO, MARCELO MACEDO; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. Effects of glutamine, taurine and their association on inflammatory pathway markers in macrophages. INFLAMMOPHARMACOLOGY, v. 26, n. 3, p. 829-838, JUN 2018. (16/16463-8)

OLIVEIRA, DALILA CUNHA; NOGUEIRA-PEDRO, AMANDA; SANTOS, ED WILSON; HASTREITER, ARACELI; SILVA, GRAZIELA BATISTA; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. A review of select minerals influencing the haematopoietic process. NUTRITION RESEARCH REVIEWS, v. 31, n. 2, p. 267-280, DEC 2018. (16/16463-8)

LIMA, FABIANA DA SILVA; DA ROCHA ROMERO, AMANDA BATISTA; HASTREITER, ARACELI; NOGUEIRA-PEDRO, AMANDA; MAKIYAMA, EDSON; COLLI, CELIA; FOCK, RICARDO AMBROSIO. An insight into the role of magnesium in the immunomodulatory properties of mesenchymal stem cells. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 55, p. 200-208, MAY 2018. (16/16463-8)

DE OLIVEIRA, DALILA C.; SANTOS, ED WILSON; NOGUEIRA-PEDRO, AMANDA; XAVIER, JOSE GUILHERME; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. Effects of short-term dietary restriction and glutamine supplementation in vitro on the modulation of inflammatory properties. NUTRITION, v. 48, p. 96-104, APR 2018. (16/16463-8)

ANTUNES SANTOS, ANDRESSA CRISTINA; HEBEBA, CRISTINA BICHELS; HASTREITER, ARACELI APARECIDA; DE OLIVEIRA, DALILA CUNHA; MAKIYAMA, EDSON NAOTO; POLISELLI FARSKY, SANDRA HELENA; BORELLI, PRIMAVERA; FOCK, RICARDO AMBROSIO. Exogenous glutamine impairs neutrophils migration into infections sites elicited by lipopolysaccharide by a multistep mechanism. Amino Acids, v. 51, n. 3, p. 451-462, MAR 2019. (16/16463-8)